Evaluation in mini gutter on s. damnosum s.i. iarvae of news formulations of bactinos (solvay)

Iil i ---l-----i--- i -; EVALUATT0N trH I{rNr-TGUTTERS; oN I s-, DAtlNGttH S: L , J r' ' '*L!,ARUAE oF NEw ronuu[nrror.rs oF lenCrl-r.os-'tsolvnll' ill .iilrjil 4, Technical Report OCP,/IRU 90.04 Insecticide Research Unit Onchocerciasis Control Programme Programme de Lutte Contre I'Onchocercose World Health Organization Organisation Mondiale de la Sant6 Bouak6 - Mai 1990 ( J'J I Wor]d H.rlth Orgrh{zrtlon - Onchoc€rcl.slt Contro'l Programn. - Ine&tlctdc Rc6€8rch Unit ocPlIRU/90.01 Page 2 ;i F ii ' ,l 1i ;l "f t ''i t, ;i 'l il i ,1 ? iri.$ii I I $, r1; liH'i I Soub16 using S. sanctipauli s.s rlarvae 1 from the , Lgwer Sassandra ffiver inSouthern C6te d'Ivoire. All the I forrpulatlons mixed;eabily hnd dispersed urell in water to form stable homogenous susgiensions. The LDSO'of tfre 2 most effbctive ofthe 50L samples were 109 mg/L/s and 12O me/L/s for Lot 436 and liot 446C respectively and iwere comparable ,to; current,operatio4al, forrnutations+for the '2 of the most effective of lthe o.1L'sampleis ,wbr6 78' mg/t/s fihe UD50 . land t122' S r r!, E and BE .i p t. i t m EV lv Du har in ds f e CAC v as the operat ona B.t. H14 rmulations in OCP. RESUME EVALUATION EN MINI-GOUTTIERES DE B FORMULATIONS DE BACTIMOS (SOLVAY) SUR LARVES DE S. DAMNOSUM S.L. Auteurs: M. Ocran & C. Back Suite pu5 tauts niveaux d'efflcacitd en ryrini-gouttiBres de prototypes deBactimos proitults en'1989 par.la firmL isoivay-oriphai, 'belle-ci et oip seir$nt'mis'd'accord pour poursuivre l'6valuation de la production industrielle. La firme afourni 8 formulations A OCP..Quatre 6chantillons (50L chacun) ont 6t6 fabriqu6s ' r dans de gros fermdnteurs -de p0:m3. Quatre autres 6chantillons (0;1L c,hacun),ont , 6t6 fabrlqu6s A I part dans des fermenteurs de 6 m3. Des bioessals ont 6te . I effectu6s d la station 'de I'IRU i Soubr6 avec des tarves de S. sanctlpauli s..s dela riviAre SaSsandra, au sud-ouest de la COte d'Ivoire. Toutes les B formulations avaient une bonne dispersion et les suspenslons 6taient hpmogEnes et stables. Les DL50 des deux dchantlllons de 50L les plus efflcaces dtaient de 1O9 mg,/L/s et :: 12O:mg/L/s pouriles Lots 436 et 446C respectlveme.nt, et dtaient comparables i la, "' j-.ir'r'./: ,: productlon' commerclale @Uramment r utllls6e' A OCP.i Les DtrSO pouni 2'. rdesr r dchantlllons de O.1L 6talent de 78 mg/L/s et 12 n1/Lls irespectivementlpour.l les. ' Lots F442 BE et F446 BE. ', Les produitlons lndustrlelles n'oni aonc pas att6int tehaut nlveau d'efficaclt6 des 6chantlllons prototypes. Solvay-Duphar a.,.1.'intbntion.de poursulvre l'am6lioration des formulatlons, les ptus prometteuses. ,, tThla docr"cnt does not conatltuta a fornr] pub'licetlon. It thould'not ba rayl.w.d, rbttrrctcd,quotad or trrns'lrtad xlthout tha rgracmant of t{or]ct HorIth orgrnl:rtlon. Authom rlono lre ratpon3'lblo for vl.ra axpra3a.d ln alEnad rrtlc1.t. l : 'i 'ii il il il I i i l t f I I i 'r) I Ir I la Control Progranm. - Ins€ctici ' ', ]; ;ri' i'i: ii"'ll :;,,- :;' ; ,.'l; il t,r ' r; , :- ii :. ,l ., i ', I OCP/IRU/90.0il ,; jl : ;t"' ' ,l .: { EVALUATION ON'S.; DAMNOSUM S.tJ. :,"i,| 1LARVAE OF 8 FORMULATIONS,i "t i .i-tj,i .,1 .J iItitt ,li'i rl : ,l ,l { I I I 1 1 .II I i "+ I I ,1 I I ll, ;s,yqP\r VAY;-DUQHARl ij:i::l 'tr ;rilllrii,l I lD'iB +4 Y qo! I i,rl : ij .1.1) OF : .i jlil ,l a .tl ll-ri , 1 ;ii r!_ , irlype]l +" i Hvi tr{e I t i ,ti"if.,,{l it. ,j; ci nt jiit,r tl rl t- of ul I tLit P ak I w T I cjc. G.E., Bo a tfr"f^n !'gtn L), wei'er tested mini-igUrtterl iV P dt i B. Ri ( ini th rr I e t g'sL e1 effi,cagvi of p pf 1 tfege ' 74 and' 127 mg/l/d I t{en developmenti and in,Apriil ( 99O i i-- 1,' lar ge fermentors and inil' strial ) J*"t*i*^+ t SU b mitte i d to OCP, along du I wt th4 _ , *_ __ -*_-,.*:. ---i.-.-.. r_+..i+- iexperimental samples of 0.1L. This report presents the results of their evaluation in mini-gutters. i. 2. MATERIALS & METHODS Solvay supplied the following B.L H14 samples from large (OO m3) fermentors: {r.s..+{tt-+.r. .*.*?--..,="ii*'-r*--.*.*.-1.^-li;.BaCtlmos-.Lot 446D.,manqfigttlfed.04/90 ' ; 2. Bactimos Lot 436 manufactu red O4/9O Bacti 446C manufactured O4/gO ,*\..- .1 -T.. .l . .\ -UZ 'manufdctured 04190 Bactimo.s Lot F446 ;BE manufactured . '-\ "1 aacti mos'l-ot faiztbEYianti?actu red .: Bactlmos .t-g} F444\BE. manufactured Bactlmos Lot F4il6 :'BE manufactured ,i "r.--+- ,*.. -+"- ,h, . ,.!.*-in{- .l . .--., r .*{--J'- r - These were firovided as 50L samples each. ; I I - t' 'oit-..,- .-,"+--.$ " - *.+-+--i*{-, ! The other fogr formulations of about 0.1L each, manufactured ln small (6m3) ferrnentors were: ;:'* i ,-' t ' 'j ';-.; L ; 1. ..i ,! ,'l ?, ,: 3. i 4. 04/so o4/siot 04/so 04/so The tesis 'were performed at IRU fleld statlon at Soubr6, Southern COte d'Ivolre wlth S. sanctipauli s.s larvae collected from the Lower Sassandra rlver. The tests followed the OCP protocol for minlgutter testing (refer to Annex for test method) for 8.t, H14 formulations. ,{ ii ucTlot'lI I'lrhlii I ij il.iIN lLrborutolr. Gontr.l lolvry, rl,l. d. Rrnobc.k 310 - ltzo lrurt.l3r E.lgium E i I I rl. 1 rlTl I I { r't I I I,. l ,1 I I I a world Hee]th Orgenizatio{1 - Onchocerciasis Control Programme - Ins€cticid€ Reaearch Unit T li ,l ts, m 4 ocPlIRU,/90.0,1 Pag€ 't n g n e v. li i Iti, ; ir;tii ri t ,i ab rj lr .r '11ii' I I I tl i; I I ir ",1al D u h re d: i; It p rth ih itn,l m9/L/s g t are SO l- lg. LD99 jb resp ffici Res fo smal w ith D5 LD95 and L Fis The LD50 ples ofi L eac are lated.-5y p bit lan aly, samples ran bett["/een mg/l/s tor les F442 t sanlie -lel BE land r! i I I lef D9E of tfe, 4 and 11338 p++b eE I I I hl LD99 be en 261 I Lotst,*.bper 442 ron BE an , lform yiless latior ci t re I I I : h-** bnt qt thei LDeg Ilil''l I i r el. '\ "| t I It ynlni-!utters, only one forinulatipni hccedtaUtd level df activity,l and bo0 i received from Solvay-Duphar and assayed in ILol 436, could be considered as having an ld bre retained for evaluation,iniriver tests. i From the foui soL iampl ' retany hlter , ative to I6s' ,n rate, this formulation can only be considered as an equivalent current formulations. Solvay-Duphar intends to pursue the ,tl rqgrp potent operational, formulations. The major obstacle remains ttrrit the expeFlmdnthfteiel 'to+the lndustrtat -tevelr : +.- . r i.- 'l ;i I -,.r;-,t-,*t ,i I 4. CONCLUSION &l RECOMMENDATIONS I I I l I I RESULISI't, l I I I lr3. 50L isampl r"t"4 Q[ p esi rhnged nd I t{ nd i te.d afo42 m S r sam e 436Ss/L/ p e 4 e t1 lons- *fJ,ifa tots LL? !i !- "'" +*# {'3$''q+.i4, j1t 4 tests i I I L d of and L rJor /s I the loxl p i d n w hi are al dt l il bi v e$ .f ,oJ., avl -i^,r 7.t. ' . :, .'; . l . i r'ij ' ;: ri : r,r; ;i;i:i:. ii liii i :,I | ' ' : ' :i, ii ,,r,^tol. ;; .,r coMPABAirvEREsul-TsoF,BoA,ss4ysoF4BATcHEsoFBAcnMosB.r.Hr{(soLvAr.)ii iI I. I i I :. :INITINI.GUTTERSONIJIATURESMUUUMDAMNoSUMTARVAE li FORMUI.ATION ltiiJI , ij .H, Jf,.',$ -fi :1 I I tl l; t, ,lt rll TEST E i,; iil cool ;ij{t$' ll 1 l Nbr 4 j'l I BACTIMOSltl LOT',a3o (soLVAY) (rRU BeO28) fl tlti ,l lt li 1 5 3 4.3 r1rr il ilr li ix s.z I 'tiqil tli l, I -T--t-rt;, I rd,-, Ir(*il i I t I e$) 6 l"ri.l' i t1,2 li(4t) I I liiI rosi(rqn7) I l LD50 c. t, ii ) 760 i! 1l t ,i, ii$ 8508. )jr f I J* LD95 mstu, l i,rrI!ii5271ill(Arc{rl u)s mglUc il t I t,?3l12lr t-],ri BACTfMOS i LOT 4/tGC (soLvA\r) (rRU B9O2a iu;1,. il I i i(37V , I ' t5.7 (271) i l I 15.2(s4 s7.o(eq 1n(-) (-t I &2 t-) lrll p00 l It i iercnuos ..ir-..r} ,. I -.L *.r" ' LjoT'4,,,2 : (soLVAY) (rRU BeO2e) 2 li!ll--r i tJtG-8G}llr t I i,.. r.| 2.1(u8) .+*J..I 8-9 (218) I(ry) i ,t8.5 eeq i r.. t zt8( -a1) I -i-,,1, r i rtboo : tzTtaet -,i -l ;";{ 1780 i I + ! (127t27s)) I i.*{^.:t t, I. I li ll I r-*l-4*dlltj r : Temp. Water - Z71C , : i-+{'",+ pH =t1-i " u -l*** ! '*i ! t-'j: Turbility = lO JTU Cbnartfry = 94.6 r.6/cm I : ' Heterogeneity too hagh to ellow catcuhtbn ol CodtUrlrrce'lntcrvet *q...j ri I -r\\ J .E o 'Ii, .i.,.{.* I I I I 6 '6;.+ +' .-t-Q --.r.;' o9oo ,i 1 000 Fig.' 1 MfNI_GUTTER RESULTS Scqtircs - experimentol LoT /+46C ?" .,*,q.l"'t"f--l+ t- ,t i:1ooI ', 3 I 1 1 I +.j 4r,+.!.-.f-r.,,-...i +1 ,"1 .*;. :5-.+1 itl i :,.. I ':l t -4F*hl* 10 LOT 4r16-D LOr .36 ,.,1L,.^,- LOT 44? I}6 MORTATITY FOR I,IATURE I.ABVAE;' iii -{i. ,i ir rruii fi il ri ii " :ifll:irlo,,:li: I i :,,i t- :l t-*,*.i 1,- I . ', + TABLE 2. COMPAFATTVE RESULTS OF BTOASSAYS OF 4 DIFFERENT BATCHES OF BACTIMOS B.t. Hl,t (SOLVA\') IN MINI+UfiERS ON MATURE SIMUUUM DAMNOSUM LARVAE WITH TEKNAR HPD FOB COMPARISON lje$ii l, li f ,-,.- i'-.1. ..r -.i '- BACTIMOS LOT F 436 BE MG-90-r7 Temp. Water -?8oC pH = 7.3 Turbidity = 10 JTU CoMnctivity = 99.1 t€/cm ' Heterogeneity too high to allow calcuhtion of Confilence lnterval t ilrl fis. 2 i"4lNl-GUTTfR RISULTS 1 00|]0 r ooo roo ) o e BJ I I I I 0 a3 I I I I o I 8oc..imcs Formulotions .i , q ..1r-,,, ]F.,..I ,r,a . r- -i 'r I 1 i I Ii I I I +'-"' r-.. -'i-.r .rl.-.'r-l.t',e*1i-,'1.+,i -. , -*il_, ... FORMUT.ATION ,| : li l ril t TEST cooE lrlr , r ill-.r+i '16 MORTALITY FOR MATUBE I.ARVAEi,ir ,l (Nt LD50 mglUc (fiq6c.t.l Lj!,? iLD95 mg/Ur (wq6c.t.) li ,, LD99 my'Uor (6ortc.t.l .j i ,ii .!. Nbr of Tests BACTIMOS ,1, LOT F 446 BE 6 i I MG-90-tI r i:l:i 3-O (716) 12.7 (852) 46.0 (sso) 89.'t ,l (+11) ,12.(n-t6i, 336(21-750) i 511:(313-1452) I rli EACTTMOS LOT F 442 BE iiir MG-90-18 ii ll I g2 (st 1) i 73.3 pon s.5 (356) 7g F3-eil Ii 261 Feo-*t) t,. 1A ($t-532) 1 BACTIMOS LOT F /T.g BE MG-90-19 o.0 (3"3) 5.5 (Nl) I I I I 3.7 39.5 (357,(290) 313 (277-W) iI t338 tita-be1t 2570 (1717-1ffi) t 2t7 i (szl) 6.6 t0.3 lstz) | (]te) 75.1 (3fi) I I I I l i res ,'( - ) 586(-! 938( -) I 'I o' LOT F 446 BE LOT F 442 BE LOT F 4ztl+ BE LOT F 4J6 BE -r,.. i I '- . t, t,t --- . TABLE 3. Results of mini-gutter te6ts at Akakro with elperimontal formulations of Solvay LDsO LD95 mglUs. mg/Us mglUe (96,q6 127.O (e1 LD99 FORM t.) 890t28 3l11 0/89 s)_2 99.2 (213) ' Corwersion in mg/Lxl 0 min ie made by multptying the vakrc by 600 " Heterogeneity too hQh lo allow calcuhtion of Confidence lnterval 9.2(-) 58.,1 -) 125.6(-, g/fTtu,1< ; It q -i I I Iq I I I I IJ 1000.) 1)ty) 1 rt) 10 690 10A 490108 ^!<AKRO '1/10,/89 C. O1/11 r9o1 2B/aa 890204 OATE TURBIOTTYt' litl F.OR t $ 2Q 890toA 3rrJ0r@ &, 7t.3 (214 97.'3 (zJt) re.0 (tet) 1@ (t6n 15.7 ( - )" @.7(-) 96.6( -) 890108 i I 31n0/@ ,m I 90.3 (2oo) 98.3 (t8t) 100 (teo) 100 (167) 9.s (2.7-t5) ,10.5 (31-5t ) 73.8 (Gt45) n 88.1 (7e) 90.r (25e) 98.6 (218) 1 m 7.8 (t.7-t 1) 55.7 (43-7t )(Til) 800204 olfll/E) t5 80.2 (tet) s7.0 (ffi) I I irsa RESULTS OF PROBIT ANALYSIS 5 lteretions CHI-2 CRITERION = 0.1HETEROGENOUS OATA 'log LO99 = 1.58629 0.316 < < -11.082 , 1099 = 38.57376 2.069 ( ( O.O0O Iog LD95 = 1.15173 0.11r1 ( < -9.083L095 = 1a.18182 1.3O1 ( ( 0.OOO 8rg. 4 rT tB38: r9et93B9 I Iintercept I = {.839,slope b = 1.568 i oecF= ZCHI-2 = 12.763I Probqbility = ,O.0Ozi HETER = 6.381T-VALUE= 4.3O0 GFAC = 1.t183 TITLE : GgOOil2'lO B. tgUBTITLE: KOPERAGUI, R ooBe units:MG/LContro] : 1.0 x -O.398 ( < -tl .23OO.,tOO < ( O.OOO S.E.b = 0,176 H1't (BACTIMoS) {'16-0 (SOLVAY) IRU 89026 SASSANoRA 24.O'1.90 DATA TRANSFORMS CORR. CORR. PREO. CHI-SQ. DEAD PROBIT PROBIT CONTRIE l ) CORR PERCOATAI 1 2 3 1 320 251 471 278 23. I -0. 39811.2 -O.699,3.2 -1.0004.3 -1.301 1.260 43.732 32.947 3 3. 168 2 DEAD 76 2A 15 '12 0. /t o.2 o.1 DOSE TESTEO l9€8424D g.t. Lr14 (eADTI'ios) 146-c (S0L!mi') IaU 89026 racFEifGJIr R. SAgStt;A 24.94.93 x oBs 215 713 270 798o5 LOG DOSE f 73. 5 25.7 10. 4 9.3 o 3 2I ,1 10 2 , 0.35 o. o1 tl .58 7 .81 F d cro z E v) IoU c( 7 6 I 2 irt I I is: Ir9e I I I 7E I J* I I .ire I I I '] I I =1 o/- -a -f I LJC C4SE ( rn ltCr. ) 0 -ttPL , Ji -\ ! iteratrong .HI-2 CPITERIOTI: 0.1 1,rS lo? i-'or-ont r-t n-,1 -0 05637 o.878263 : -0. ?5615 : 0 5(dO55 2 515 281 000 -o.125 < ( 0.023 o.7n8 / ( 1.065 -0.310 ( < -0.1920.r89 ( ( o.6a? I noo - t-,099: :t95 Ln9 5 -n 71934 o 'p.24R 7.51P ? dct -0. 757 ( o 171 ' ( -c. 71 r 0. r95 t-. S.E.t = C.159 DI!F: CHI-? _P-.hab i I.i ty : HETFR : T-\/Al.l-rE: ItrAC: ? o 'I '1 0 Bo EF a9 E cr a: ar oo9 960 rrTt_E no^.ld?tE B.t 9llRTITT tr. yootrqAGUJ . p DoSe trnits r!C/r- Cont-or ?.7 1, H14 rEqeTJMOS) 436 (SOl \,'AV\ rprr B9o:P SASSANOPA 21.01 ?^ NATA TRANSFORTIS ^actr Trcrtrn oEA! ^oc 332 55 rqq <oQ :3'r 38 dc 1. r oG ^oapNNSE !EA^ anaP PQF^ acI _so DAOBJT oAa?TY a.ilTRro oFoar0i6{ '61 '{9 :99 , 1?-d rrl ? ^.1 -'-.!-:E a t, r1'- I.:i: -,-LJ: ?05 136 ?3c n1l '9ofTl''f':' 1l; ,an 'ILr,' Irrr :,rr:c, . l't. l, -',-'r--, :i.!1. i: 5r),9tla1 I E ?e l4n" !1f. ta ,4t ,i, /tt hil O/r 4 :- u- o r c( Fr.g. 6 RESULTS OF PROBIT ANALYSIS 5 ltcratlonB Iog LD99 .= O. LD99 .= t .3 t2916 t|6351 CHI-2 CRITERTON = O.1HETEROGENOT'S DATA -O.3'10 < < 9.1210.t|57 < ( 263'1089156.926 -O.'183 < < 6.681 o.329 ( < a80t674.331 -1.115 < ( O.3570.077 < < 2-278 3.E.b = O.138 1 L09 LDS og OEGF CHI-2 -O. t 1 3a9 0.7700ao 2 37. 31 9 o. ooo 18. 660 -2 5=5= ii log. LD5O ,= -0.6991 4 LDSO '= O. 19992'l tntnlrcopt e .= 6.964llop€ b.= 2. Bo9 I ?robabi I ltyI HETERT-VALUE= 4.3OO ' GFAC = O.832 TITLE: c9OO'121A B.t ; SUBTITLE: KOPERAGUI, RDose units:MG/L I lCcrtrol ,: 2.7f :l,iil DATA DOSE TESTED DEADI c300421q e.t. t K9F H11 (BACTIT.TOS) SASSANDRA 24.0 a46C (SOLVAY) IRU 89027it. 90 DATA TRAIISFORI.I9 LOG CORR DOSE DEAD r,t4 (8ACTI'10!1 41€C r54! Lh,i ) !Ftt -)gc2l , R. SASgt|JAA 34.04. 13 ,t CORR. PRED. CHI-SO PROBIT PROBIT CONTRIi x 0Bs CORR PERC ,"ii ,l ll r99 I I I JQC J?0 t-^J/t) 86. 7 43. 613.r 8,7 72 a1 37 84 I 5 7 ttl 846 000 155 310 5 5 1 3 111 839 890 639 87 15 15 11 o. r 0.2 0. t o. 05 1 2 2 1 321 s92 271 377 28? 't7 7 d3 42 o -0.3982 -0. 6997 -1.00c1 -1.301 280. 8 6 171. O 136.6 332.7 3 B i I I I ?) I I I .t "l o,, t.t; )l /i/l I i I T 1 F @c ato llJ tl, c o .t' 18 q 'i ! I I .t I .l I I I I f trrII I I J I 3 ? -4 2€ LoC DGE ( rr' HC/l- ) li n Fig. 7 RESULTg OF PROBIT ANALYSIS tl iterations CHI-2 CRITERION = ]og LD99 = O.17221 0.326 < ( O.669LD99 = 2.966'tBl 2.117 < ( {.665 'log LD95 = O.22556 O.110 < ( O.380LO95 = 1.680978 1.288 < ( 2.399rl , 1og LO50 = -O.36982 -O.{18 < < -O.31OL05O = 0.126752 0.382 ( ( O.490 9.E.b: O.211 o.1 CORR. PREO. CHI-SQ PROBIT PROBIT CO}ITRiB .l )l int6rcept!lop€ 6.O222. 763,t b ,l t o 1 I 0 DATA DOSE TESTED DE O. '3 261o.z 290 o. 1 248q.os 328 128 65 22I a .902{. t16 I aa) ?.o37 1.922 4.091 3.259 2.127 0. 07 0. 09 0. 23 0. 81 2 190 5{9 oo0 960 o22 4A ?2 o 2 1 2 a 1 DEGF CHI-2 Probabi l ity HETER T-VALUE= GFAC = TITLE:c90o{zac 8.t. SUBTITLE:KOPERAGUI, R. Oose units:MG/LContro'l : 4.4 I -? H1'l (BACTIMoS) 'ttl2 (SOLVAY) IRU 89029SASSAN0RA 24. O,t. 90 OATA TRANSFORMS AD x OBS LOG CORR DOSE OEAD CORR.PERC.f 5 1 9 1 n6 18 I 0 21 .7 54. 6 11.6 0.5 398 699 000 -0 -0 -1 -1 1I 7 2 js: I -?t jic I I Jto I I Gt0043ec e,.t. H't4 :e€TI"0s) ..? (S0t_hyj) Ipu 99e29 l:oE ti4JI,. R. i SAeSrdl:A, 24.04.93 301 LCC DGE ( in IIG/L ) {I 7 bF d clu U a c oa L I I I 4i I I l q 3 2 I { 0 2 lrl i llI{ rig.8 RESULTS OF PROETT ANALY9IS a lteratlons CHI-z CRITERION = O.1HETEROGENOUS DATAlog LD99 = -0.06985 -0.283 < ( 0.384LD99 = 0.851tltlO 0.521 < < 2.421 lo9 LD95 = -0.25119 -o.'120 < < O.O97LD95 = O.560808 0.380 < ( 1.25O ,-0 ro. l l S,E I "to8s 3 2 o 6 o-1 o.2 o.1 1o9 LD50 = -0.68887LD5O = O.2O17O7 .786 < ( -O.560l6'l < < O.275 , lqtercegit slop€ It= b,: DEG} =CHI-2 =Prob.b{ Iity =HETER =T-VALUE= , GFAC = 7. 589 3. 758 rl b= o.rlo 1 2 t 3. 039 o.001 6. 520 tl .300 o.145 TITLE:G9ooil25B 8.t. 9UBTITLE: KOPERAGUI, R.Oole unitB:i{G/LContro] : 1.6 f Hla (BACTIT{OS) LOr F aa6 BE (SOLVAY) SASSANORA OATA TRANSFORMS DATAI OOSE TESTEO OEAD E9m{259 B.t. r{14 Iros) LoT F 446 6E (S0LVAV) , R. SASSATDRA 1t1 550 852 716 397 253 to8 26 89 16 12 3 396. 2 6. 239 6248.2 {. 878 I95.9 3,7A7 3lil .8 2.960 2 093 962 831 699 26 18 5q 72 89{5lr ? 2 1 ? 't 4 -O.398 o -o.6997 -1.O006 -1 .301 LOG DOSE CORR DEAD CORR. PRED. CHI-SQ PROBIT PROEIT CONTRIi CORR PERC 1 2 3 4 o50 I 7 6 J 4 3 .t99 I I I .+99 7E F q d o, Z U o d .I I I i 3E 1E a 2 -4 -? LCC t[SE ( rn ltC/L ) 0 ? Fig.9 .14586 7 1 a732 -o 0. LD99 LD99 og RESULTS OF PROBIT ANALYSIS 2 itorrtions CHI-2 CRITERION = 0.1 -o.221 ( ( -O.052 o.602 < ( o.887 < < -o.296 < < 0.506 -o.913 ( ( -O.858 o.122 < < O.139 3.8.b = 0.189 LOG CORR DOSE OEAD lntcrcept a'lope LD9 -o.362320.'t34t90 log LD50 = -O.88r177LOSO = 0. t3O38. 7. 786 3. ! '18 DEGF =CHI-2 =Probabi I lty =HETER =T-VALUE= GFAC = TITLE: cgOO.t25C 8. t SUBTITLE: KOPERAGUI, R Dose units:MG/LControli: t.O t -? H1{ (BACTIMOS) LOT F 1.2 BE (SOLVAY) 3A33ANDRA OATA TRANSFORMS . tll6 38{-0o. a- 5= a b 1og LOg I o.224 0. 652 I .000 1 .960 O.0l tl DATA DOSE TESTED DEADI 1 0.{ 336 3142 0.2 307 2?53 ' O. t 51rt 186 x oBs CORR. PREO. CHI-SQ PROBIT PROEIT COI'ITRIi. CORR PERC 93.5 -0.398 73. 3 -0. 69936.2 -1.O00 53 58 63 313 221 182 ? 5 7 I 2 7 6. E 1. 5. 506 5.611 4. 629 o. 07 O. 1il 0. o2 93. tl 73. O 35. 5 G9S|25C E.t. H14 (enCTIrC9) LoT F 442 BE (S0LVAV) t0PERACIJI, F,. 9r{SSA}OAAI I ,] I J F a & =U(l) E oQ F .f 3 6 2 I I +'tFt'- J I I .t I I ') -4 ?0 LOC DGE ( rn l{C/L ) ,l Frg. l0 I ,l I I I interc€pt a =E1ope : = DEGF = ' CHI-2 =Probabi I lty =HETER =T-VALUE= GFAG = 5. 718 2. 516 2 3. 533 o.168 I .000 1.960 o.023 TITLE : G9OOrt25E B. t.gUBTITLE: KOPERAGUI I R.Oose unita:MG/LContro'l : O.6 I 3.E.b = O.199 RESULTS OF PROBIT ANALYSIS 4 iteratlons CHI-2 CRITERION = log LO99 = O.6318{ O. 'l6tl ( ( 0.859LD99 = 4.283945 2.911 < < 7.23O ]og LDg5 = 0.'36{14 O.231 ( < 0.541LD95 = 2.312820 1.7O2 < < 3.{99 'log LDSO = -O.28198 -0.336 < < -0.212LD50 = O.522116 0.a61 ( ( 0.Eia 0.1 CORR. PREO. CHI-SQ PROEIT PROEIT COI'ITRIi H1'I (BACTIMOS) LOT F .I4. BE SASSANORA 25. O't. 90 OATA TRA}ISFOR}IS a OBS LOG CORR DOSE DEAD CORR PERCDATA T 1 2 3 1 357 tt90 291 323 7210.4 o.2 0.1 0. o5 DOSE TESTED DEAD , d900{258 B.t. H14'(BecrIH0s) Lor F 44. BE K0PER*UI, R. SASS.+IDRA 6.U.95 LOC t{SE ( rn NC/L ) 141 67 16 ? 39. 5 -O.39813.7 -O.6995.5 -1.OOO o.6 -'l .301 3.880 3.3t17 2.O12 /t. 705 3. 938 3.172 2. 406 39. 7 6tl . tl 1{.3 0.5 13,1 4.9 o.2 o o 2 o 39o8 65 11 70 i l I I I I I I 7 6 , ,95 J l€r I , t-C 1 F qj &o @ c 3E -llF .iEt' I -i I I 3 2 -4 0 ? ,{ I t r9.. l.I RESULTS CF PFOBiT 6 iterations CHI-2 CRIIEQIOTI = 0.1 HETEROGEIIOUS OATA -0388<1-2.?86 o.409(<00c5 -0 491 (0 323 ( AIIAL'/S I: 1cA LD99 : LD99: c.194071.563401 'log LD95 = -0.010121.D95 = 0.976974 1o9 LD50 = -0 50:94LD50 = 0.31d091 IRR FqR 1 OBS LOG DOSF CORR DEAO 868 1 ( -1, . 0.01 i nte rcept s lope b= 6. 6793.338 3.E.b = 0.153 1. L ,R DEGF:CHI-? =Probabllity =HETER =T-VALUE =GFAC = 117 o 58 4 2 2 383 000 942 300 293 TITLE.G9NO425A SUBTITLE: KCPEPAGUI Dose units llG,/L aontrol 1 0 Z DO3E TESfED OEAO 14 iBACTII,IOS) LCT F 436 BE (SOI.VAY) S ASS AIJD R A DATA TRAIISFORIIS CORR. PEED CHI -SO PROBIT PROEIT COIITRIB CCRR PEPCDAT AI 1 ? 1 0.4 o.2 0.1 0. o5 39s .1 19 5Er 823 r98 4l 37 3.'l e9?(11(r 301 197.0 39.? 31.7 13.9 5. 6EC 3.631 3.414 2.A77 50 A6 41 36 ?? 58 o 36 75 5 I 55 76 28 5343 ?? a) 75..1 1',-) "66 -0 -0 -1 2 4 6 7 :!rD+i:f: p,. t. H.4 ip€r-TItirls) LCI f 1u 3E (!oL,/tv) T:OFEEAGJI, R. SASSA}OR;] .9't I I!?': rl5 I !_- , tl- o/ o I 71 I I oh @ ct Cr U U] a I 4i o I 3l ! -'lt-i - !r_ a I € LJ: tr-r:i -r, I I IANNEX '{ji:,+:'i OCP PROTOCOL FOR MINI-GUTTER OR OPEN-CIRCUIT TEST BRIEF DESCRIPTION Thc lrini-gutter or open circuit systeo for te6tlng larvicide6 on SiDuliun dentbsqn s.l. larvae har been degcribecl by sgveral scientlsts in tho past in divers forms, with different material6, The system being used by OCPIIRU was developed by Messra Pierre Gui'llet and Henri Escaffre formerly of thc Institut sur'lr Recherche sur la Trypanomiase ct 1'Onchocercose. It is principally uaed in tcsting suspension concentrates such es BacilTus thuringiensis and some water basad floHables. It has not been successful for testing emulsifiable concentrates, except when medified to use large volum€s of wat.er. fn general, it consists of 'long and narrow metal gutters mounted on framci Rnd meta'l 'legs. The gut.-ers are colonised with larvae and river water is channelled into these to supply constant uater f1otr. The larvae Hlrerr Hell as:ablisl'led.in their new rnilieu are then treated Hith insecticides and mortalities are calculated accordingly. TECHNICAL DETAILS In detail the mini-gutter device consists of 3 parts: a series of 8 metal gutters or troughs each rncasuring 1.5 m long by 7 cm hide ancl 3 cm deep all painted white and mounted in paralle'l on a metal frame that is provi,Jed }iith metal 'legs each :rbout a metre tal I. The assernbly is insta'llecl alongside a river barri, slightl) inclined at tlre ioHer (open) ands of ilie gutters and pointing downstream. A rectangular collector tank of aLcut 30 litres vo,ume *ith I individual tap openings at the -baE€ of one of i.ts brgadsides is mounted on metal lcgs at the upper end of the.series of gytter-s. The nozzles of the I taps are than inserted into the upper ends of lhe gutters. A seri€s of bafflcs buiit at the upper end of the gutters is intended to mix the solution of insecticides f'lorring in the gutter during t,he time of tr€atment. llormaily water from th6 river is channe'lled into the co'llector tank b, Sr.vity :low, passing through tl-,e taps and into the indiviCrrdl gutters. Ttre collector tank hes an overf'low and acts as a constant pregsure head, ilGq6J.4 -4i -t Lr^-i n*-.t,',i*,io.l p, .-+ .,- i g-i..;1,,i -3' i i**i t '+-,?...i $.F-TREATMENT PROCEDURE i"-.1 L "g f+ ;* t-{+ + i .qi ii r For testing larvicides, larvae are collected from the river itsc'lf or from a nearby Dreeding site and dre carefrrll/ detached from their natural supports into the gutters, y{here they reattach firmiy, and are left urrdisturbeo for at least 3 hours before'iarvicide solution is addeo. Before traatment, insecticide formulations are freshly prepared by pipetting an aliquot of the 6amplc into 1 litre of river Hater. This is then serial'ly cti'luted by taking 1OO c.c. of this into another plestic bottle contrining 9oO c.c. of rlver rater.i This becones the second so]utlon from which exact vo'lumes 816 pipett€d to treat each gutter according to its discharge ancl the concentration required. The volunre tJ be applied'in each gutter- according to desirEd conceiitration and measured clischargB is read fr<xn a tHo-nay table. Th€ larvicide is applied in 10 nin. A 'l .5'litre plastic bott'le cut off at its basa and fitted at its nouth with. a rubb€r tubing I Dm in cliameter at the tip of rhich is attached the nozzle is . invcrted rnd placod on ar6unted fiane" attached to th6 co]loctor.' This sirvcs aE th€ appr..tus fi. " dclivering the insecticid€ solution into each gutt€r passing first over the baffles to €nsure good nixing. ThiB method comes close to simu'lating field treatment conditions. Tho numb€r of concentratiorrs t€sted range from 3 to 5, with at least onc replicate each. Ltrvae d€teching frofi the gutter are caught in nets attach€d to th€ ]ower end of the guttcrs. After ztt h dead and Iive larvae are col'lect€d, sort3d and countect. The clata, correctcd for control mortr'lity, tr. aubnittad to atend.rd Probit ana'lysl6. -l