Medicinal plants in therapy

11/ e /1 tpublcand up-to-date survey of the concis et fiable de la situa- a present position in the se- tion actuelle dans le do- lected fields, and, over a dmaineconsider. Des experts / r / / ~~~~~~~periodof years, will cover / couvriront ainsi successive- / / / / ~~~~~~~~manydifferent aspects of /ment de nombreux aspects des / Xrn" ^ ;" t / / ~~~the biomedical sciences and /sciences biornedicales et de la / e/v nGF& public health. Most of the / sante publique. La plupart de / / / / ~~~~~~~~articles will be written, by / ces articles auront donc ete / / / / ~~~~~~~~invitation, by acknowledged /rediges sur demande par les / experts on the subject. specialistes les plus autorises. Bulletin of the World Health Organization, 63 (6): 965-981 (1985) © World Health Organization 1985 Medicinal plants in therapy* NORMAN R. FARNSWORTH,' OLAYIWOLA AKERELE,2 AUDREY S. BINGEL,3 DJAJA D. SOEJARTO,4 & ZHENGANG GuO5 One of the prerequisites for the success ofprimary health care is the availability and use ofsuitable drugs. Plants have always been a common source ofmedicaments, either in the form of traditional preparations or as pure active principles. It is thus reasonable for decision-makers to identify locally available plants or plant extracts that could usefully be added to the national list of drugs, or that could even replace some pharmaceutical preparations that need to be purchased and imported. This update article presents a list of plant-derived drugs, with the names of the plant sources, and their actions or uses in therapy. Since most medicinal plants occur naturally in a large number of countries, a plant of potential importance in one country may well have been studied by scientists elsewhere. Considerable time and effort could be saved if their findings could be made available to all interested people. Pooled information is especially critical when it comes to drugs, as a value judgement on the safety or efficacy of a particular drug can rarely be based on the results of a single study. In contrast, a combination of information indicating that a specific plant has been used in a local health care system for centuries, together with efficacy and toxicity data published by several groups of scientists, can help in deciding whether it should be considered acceptable for medicinal use (1). No accurate data are available to assess the value and extent of the use of plants or of active principles derived from them in the health care systems of countries. WHO has estimated that perhaps 807o of the more than 4000 million inhabitants of the world rely A French translation of this article will appear in a later issue of the Bulletin. Research Professor of Pharmacognosy and Director, WHO Collaborating Centre for Traditional Medicine, College of Pharmacy, Health Sciences Center, University of Illinois, 833 South Wood Street, Chicago, IL 60680, USA. Requests for reprints should be sent to this author. 2 Programme Manager, Traditional Medicine, World Health Organization, Geneva, Switzerland. 3 Professor of Pharmacology, Program for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, Health Sciences Center, University of Illinois. 4 Associate Professor of Pharmacognosy, Program for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, Health Sciences Center, University of Illinois and Honorary Research Associate, Department of Botany, Field Museum of Natural History, Chicago, IL, USA. 5 Research Associate in Traditional Medicine, Lanzhou Medical College, Lanzhou Gansu, People's Republic of China. 460 -965- N. R. FARNSWORTH ET AL. chiefly on traditional medicines for their primary health care needs, and it can safely be presumed that a major part of traditional therapy involves the use of plant extracts or their active principles. In the developed countries, too, plant-derived drugs may be of importance. In the USA, for example, 25% of all prescriptions dispensed from community pharmacies from 1959 to 1980 contained plant extracts or active principles prepared from higher plants. This figure did not vary by more than ± 1.0% in any of the 22 years surveyed (2, 3), and in 1980 consumers in the USA paid more than $8000 million for prescriptions containing active principles obtained from plants (4). Despite this, virtually no interest is shown by pharma- ceutical companies in the USA in investigating plants as sources of new drugs. Industrial interest in exploiting plants for this purpose is almost exclusively found in China and Japan. Clearly, the pathway is open for scientists in developing countries to organize and implement interdisciplinary research programmes for the further utilization of these natural sources of drugs. These sources are usually available in abundance and can provide safe, stable, standardized, and effective galenical products for use in primary health care or can lead to the discovery of new biologically active plant-derived principles that may be candidates for use as drugs. However, before considering how such programmes can be implemented, we must examine whether plants are a logical starting-point for drug development programmes. MEDICINAL PLANTS IN THERAPY Secondary plant principles in primary health care The drugs listed in Annex 1 have been, or are currently, obtained from plants. As many examples as possible have been included of plant-derived drugs of known chemical composition that are used in various countries in primary health care or that are recognized as valuable drugs in widespread (i.e., non-prescription) use. For this purpose we have relied primarily on recent pharmacopoeias of selected countries, on the current clinical literature, and on personal knowledge of drug use in various countries. A few of the drugs are simple synthetic modifications of naturally obtained substances. In some cases, the natural product is now replaced by a commercially synthesized product. Annex 1 shows that there are at least 119 distinct chemical substances derived from plants that can be considered as important drugs currently in use in one or more countries. In Annex 2 these drugs are classified according to therapeutic category in order to highlight the broad range of uses for which plant principles can be employed. Altogether, about 62 therapeutic categories can be distinguished. From Annex 3 it can be seen that these drugs are primarily obtained from only about 91 species of plants. Most of these plants could be adapted for cultivation and use in almost every country. Research is nevertheless required to determine whether the useful active principle could be produced by plants cultivated in an alien habitat. The economics of cultivating such plants and obtaining their active principles has also to be carefully considered. Correlation between the use of plants in traditional medicine and of the drugs obtained from them One of the major approaches in developing new drugs from plants is to examine the uses claimed for a traditional preparation. Although investigators involved in the development of drugs from natural products usually argue that there is a close relationship between a traditional preparation and a drug obtained from the same plant, data supporting such claims have not been presented. However, an attempt has been made to present in Annex 1 966A MEDICINAL PLANTS IN THERAPY a correlation between the traditional uses of some plants with the pharmacological action of the isolated drug for 119 substances extracted from plant sources. Although our studies are incomplete at present, we believe that the three levels of correlation indicated in Annex 1 are reasonably accurate. The correlations were established as follows: (1) If there was positive proof of a correlation, based on a study of the ethnomedical uses of plants and a knowledge of the actions of the chemical substances extracted from them, this was designated as "yes". (2) If there was some correlation between the use of a traditional plant preparation and the use of substances derived from it or a related plant, we considered this as a positive correlation and indicated it as "indirect". For example, Digitalis lanata Ehrh. has not been found to be used in traditional medicine as a diuretic or for the treatment of congestive heart failure or dropsy, uses that are related to cardiotonic activity. However, the isolation of several drugs from D. lanata (acetyldigoxin, deslanoside, digoxin, lanatosides A, B and C) that are currently used as cardiotonic agents was due to the known usefulness of D.purpurea L. as a cardiotonic agent. Chemical studies on D. lanata were therefore initiated with the possibility of finding cardiotonic agents, even though D. lanata itself was not used in this manner. Similarly, the "indirect" discovery of tubocurarine was based on a study of Chondodendron tomentosum R. & P. and other plants used as arrow poisons by Indians from various cultures; study of the paralysis of the skeletal muscles of birds in flight and of running animals by arrows dipped in "curare" products led to the discovery of tubocurarine. Altogether, 10 plant sources are designated in Annex 1 with an "indirect" correlation. (3) Thirty-one plant-derived drugs were found for which no correlation could be found between their use as drugs and the traditional uses of the plants from which they were obtained (Annex 1). However, more careful study of the older literature may reveal some relationship. Of the 119 plant-derived drugs listed in Annex 1, 88 (74%0) were discovered as a result of chemical studies to isolate the active substances responsible for the use of the original plants in traditional medicine. Approach to the study of plants used in traditional medicine Annex 1 shows that a fairly high percentage of useful plant-derived drugs were discovered as a result of scientific follow-up of well-known plants used in traditional medicine, and it can be concluded that this is a good approach for discovering other useful drugs from plants. In contrast, other approaches, such as phytochemical screening, massive biological screening of randomly collected plants, and phytochemical examination of plants with the aim of identifying new chemical compounds have not proved to be very helpful in discovering new drugs. However, there are two fundamental questions that must be considered before one initiates research on plants used in traditional medicine. Is it desirable to put in effort to discover pure compounds in the hope of using them as drugs per se or is it preferable to go on using traditional preparations and make no attempt to identify the active principles? For the majority of developing countries, the cost of imported drugs on a large scale is almost prohibitive. On the other hand, these countries have an enormous wealth of information on medicinal plants, which are not only cheap and abundant but also culturally acceptable. Furthermore, most developing countries have neither a well- organized pharmaceutical industry nor the manufacturing capacity to isolate large quantities of active principles from plants should they be discovered. Thus, programmes for this kind of drug development in these countries have to be well planned and 967 N. R. FARNSWORTH ET AL. coordinated (within the country), and they may be carried out in stages as illustrated in Fig. 1. This flow chart focuses on the initial need to produce safe and effective galenical products but includes the long-term objective of discovering the active principles. These programmes could eventually lead to the development of a pharmaceutical industry in the country. Critics of the use of galenical products rather than pure active constituents should consider the following simplified example, which illustrates the value of galenical prepara- tions. A chemically standardized tincture ofA tropa belladonna for use in treating stomach ulcers has a therapeutic efficacy at least equivalent to that of a standard dose of atropine sulfate (the major active principle of A. belladonna). The plant itself can be cultivated easily in almost any country and the manufacture of a stable, standardized tincture would require little in the way of hard currency, which would be needed to import tablets of atropine sulfate. Other similar examples of efficacious galenical preparations that could be promoted in developing countries can be identified from the information presented in Annex 1. There is therefore much in favour of establishing programmes for producing standardized and safe galenical traditional preparations for potential use in primary health care, as shown in Fig. 1, with the eventual aim of discovering their active principles. Even if the active principles have not yet been identified in some of the plants used in traditional medicine, historical evidence of the value of such plants could result in useful preparations, provided they are safe. Evaluation of safety should therefore be a prime consideration, even at the expense of establishing efficacy of the preparation. WHO 851626 Fig 1. Flow chart of sequence for the study of plants used in traditional medicine. 968 MEDICINAL PLANTS IN THERAPY Simplified pharmacological pre-screening of plant extracts One point that should be noted about the biological activity data on plant extracts reported in the literature is the difficulty of reproducing many of the results. In general, the more sophisticated the bioassay, the lower the chance of being able to reproduce the data, but the reason for this remains elusive. Many of the reports on the pharmacological testing of crude plant extracts have been published by investigators working in laboratories in developing countries. One explanation might therefore be that laboratory animals in some developing countries are undernourished and thus respond biochemically in a different way from animals that have a better nutritional intake. It is also possible that low-grade laboratory animal infections, especially parasitic infestations, which may not manifest themselves visibly, could cause animals to respond abnormally to the action of drugs. The inability to reproduce experiments involving the biological evaluation of plant extracts has also been attributed to variation in the chemical constituents due to the age of the plants, the time of year or season when they were collected, or the geographical area where they were collected. Although chemical variation in plants is well known, we are unaware of reliable experimental data indicating that this is the reason for the inability to reproduce the biological effects of plant extracts. Scientists are generally reluctant to accept data on the effects of crude plant extracts in humans or in intact animals unless an explanation of the reported effects is also given. Conversely, data from mechanistic studies (usually in vitro) on crude plant extracts rarely attract much interest in the absence of evidence demonstrating the effects in an intact animal or human subject. In most developing countries, chemical and botanical expertise is usually readily available but experienced pharmacologists are rare. If trained pharmacologists are in short supply or if they are not interested in collaborative efforts to discover new drugs from plants, it is feasible for chemists to set up and implement certain in vitro bioassays (some- times referred to as "pre-screens") or cell-culture systems that can provide valuable information. Similarly, pharmacologists may find it more convenient and economical to study drug effects in vitro as an alternative to using intact laboratory animals in their research. There are sufficient bioassay techniques described in the literature to enable almost any biological activity of interest to be studied without using intact animals. Indeed, there is a worldwide trend to avoid experimenting on intact animals in the early stages of drug development. Some of the "pre-screens" rely on chemical or biochemical expertise rather than on pharmacological knowledge and training and hence should be managed by chemists. A few of these bioassays are listed in Annex 4. Most of the "pre-screens" indicated in Annex 4 can be performed using relatively simple equipment. Virtually all assays can be conducted using tissue culture equipment, a CO2- incubator, an inverted microscope, a sterile hood, a cell counter, water baths, dry air incubators, an autoclave, a recording spectrophotometer, and a liquid scintillation counter. However, many of the in vitro "pre-screens" can be effectively carried out without some or all of this equipment. Thus, the chemist who does not have collaborating biologists could set up one or more bioassays that facilitate the isolation of biologically active molecules. These compounds are usually likely to be chemically complex and possess novel structures that are interesting from the scientific point of view. The "pre-screens" listed in Annex 4 have all been successfully employed for the biological evaluation of crude extracts and may need only slight modification to adapt them to laboratories where conditions are not the best. The information provided in the cited references should be adequate to set up the bioassay systems, as well as to facilitate an understanding of the basic principles involved. 969 970 N. R. FARNSWORTH ET AL. CONCLUSION Scientists in developing countries are entering an era in which plants can be expected to occupy a prominent position in the list of national priorities. This type of drug research could lead to industrial development in the country where the discoveries are made. The source of starting materials is normally abundant and readily available since in most developing countries the flora remains virtually unexploited, and we believe that over the next two decades many useful drugs will be isolated from plants. 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Use of unscheduled DNA synthesis in freshly isolated human intestinal mucosal cells for carcinogen detection. Cancer research, 40: 3155-3157 (1980). 53. SIRICA, A. E. ET AL. Use of primary cultures of adult rat hepatocytes on collagen gel-nylon mesh to evaluate carcinogen-induced unscheduled DNA synthesis. Cancer research, 40: 3259-3267 (1980). 973MEDICINAL PLANTS IN THERAPY 0 0 00 0 0a)0 _0 .!: >- >- >- > > U) C 0 C 0(3 t I C 0 E E 0 C C U) 0 .3 C E c .N 0 C 0m C(3) .0cr_ (fl ~a U) 0 o0G UGc0-) O 0 0 U- 0 Q CCC75 U) U) 0 0 C I > ) E <#> E0c_ (° C S:E S:E 000 Cz C O E o C 0 I 0. x coN. -C (3 a CE 0 U) C .10 0 aQ 0 .E Z: 0) ._ C .E c) a a CO z x at c 2s J () ,$ U)a 0Q 0n >X~ ~~0..!a a>2 qo t S 52~~-. Ct 0nmS 0 *-0 *0 .30 u C C 0 .3 -0 0 .0 C 0 x 0 0 0 E m 0) .0 _V n C 0 0 0 m L) o 0 =~~~ x - 0c c < < < < < <i <: < < .( 0c C 0 .C .E0 C -j -C 00) (3 0 (3 0 C 0 -J0 co 0io Nm 0 0 02 0 0 a. N c, crj~~ ~ N- N iC co C j o C CK 0 .@~~~~~~~~~~~~~~. 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St C) CD ~~~~~~~~~~cD Cu 0~~~~~ CC C C Cu )0) ,, E E ° @ E 1C Cu Cu m C Cu C 0)Q Cu E C X C 0 C C = ._ -C 0 0( 2C 0 0 00 0 U U) QUC C E C C u x .c 4: *3- 4) 0 c 0 0 .c C cCCuC0 C .5 0 E 0 a 0C 0. 0 ,Q ._ C N) C Cu:2 a) a) E D -- :2 *- C 9 --C NA t>>m E a a 5 6 w w lL (0 t0 974 C 0 ', M (D 0 Cu 0 CL 0 0 0 C Cu Cu 72 0D 0 o E EC .C 0c o0 .2 0 C 0 ._0 0 'a Cu 0)'aI .5 0 ECu 0 c cn -E Cu a0 CD Q) ?I 0 a CC) 0D cn to s .C 0 -- m .X .Cu. Cu n -u .~? x q) .,,I E 0 w C2Cu C -0 N CuU Cu E,a a: 6 a Cu C0) CO -0 Cu C, J Q-I Cu QL 2l0. -0 0) Nk 0 CuN 0) -S) N Cu C)0 00i Cu -i -5 0 cn C0 0 E C 0 01 C ._V Cu'- E .2 .0 0 E 0 0 0 Cu 0 .0 (D-._ cnCu N .Cu C) .5 U 0 '-E E 0 Cu E cn 0 C2 0 0 Cu C) U) Cu co N 4) -c 0 Cu cnCu Cu C CuC CD m 0 E C 0 0 V Cu Cu '-0. 0 C 0 0-i4) m 0 .0 0 E 0 ._ 0 0 Cu U C Cu am 0 4- CV Cu N C C a 0. x Cu C C 0) 0- Cu .C U 0 0, > a .0 '- = +- O C E 0 E 0 0 C V o 0 0 -o o1 0 0 o , 40 a E o lU .°~~~~~~~~~~~~~~~~~~~~~~~~~r _C 0.> Z4 .a ;- 0) .C -c0 ._ .N 0 Cu ._ .2 CD a) C .5 CD -5 ao00 CD 975MEDICINAL PLANTS IN THERAPY (3 .Q > a c c c0 00 E ~ 0 00Q M0> E- m- . C C ' C.O C >~ U) 0 m x 0o < cn<w< wOLA S m .0) . E30e4 t E _=ta UJ I co 2 IIt -C 0 0 04 co i.0 0 a. 0 03 4( C 0 E 0 C 0 C 0(3 C2 U) 0 `0 0 0 U) > .> i; *U 40 (3 ) *_ 0 +1 ', C 0 3 CM C > C C <r a z < 4 0 0 -X 0) C i jco m 11 C'0 .0 k. . o mnf-Na,1 1 0000(30 0 0 :0 -j 03 0 0.0)0. C 0 .T 0. 3 ._2 CD . 4) C (3 1 4.... o .°2 C 0. X C] 0) alO i X O( 0 Cm C x - C0 CO C) z U) ° { C0o aC c 0 Co t EE 000 W) 0a 0 CD 0 0. 2 -jOa ci 0 (3 0 0 0 E -; I E 0 zE ,Q t a to > m. 0 C E U) .O ~~~~> 0 > _ 0. ., .5o C~~~~~~~~~~C c im Co m>O I < < F m O en z G < < m _ < M 0 m. C o m >. 00 0 a 0 .r5 0 .0 .0 C LO. ml C 0 C 'o ._ c E n c 0 .0 0 0 > > I I II Y C 3 m C .C 00 0 m e 0 0 +- 00 C1m C*0 0- o 2 2 ,0 0 C 0cO 0 C 0 *0 .C 0OQ cm 0 L. C 0 z 4)C 0 2 0 2a (3 0 0 ._ 0 z 0 4) C ._ C r- ._1 0 9 m C .C 0 (a 0 z 0 0 C .co .0 0 ._ 0 x 0 CL .' 6- U) CL c 0 x 0 0v is Q (3 0 0. c .0 0 C 0 .4- E m.5 0(3 0 00. E 0 0. Z 0 0. as nL c 0 x0 .5 0 m E n-00 E Ul) U) U) a) C .5 E *C , ._ > 0 0 = 0 0.> _> c EU >> * C .- 0 E o = ccU00 O. X X ffiC 4- .C X )0 C C 0 a. 0 C 0 0 .5 X c Oa ,c 0 > 0 O1 ° 0 0. C 0 0._ 0 n 0 .° .50 D 16 0 0 (3 0) C 0 ._ 0 C 0 (3 U) C 0f c 0 .5z .5M 0 E E 0 00 u 3 0 L. 0 U) 0 z N. R. FARNSWORTH ET AL. c - 0 at <Qi- oirQ J Sc I- (Uw U31 a x'C Xi cC m- .z. RU - x c 0 (o .c a (U 01 (U C 0 C. E c a (U 0z Ct 08 a C~ (U .r .a E _ c 0 .2 V C,, (. x -J c t- m c E .In (. C') in n U '5- ._ ._ E (U ._5 ._ '5 Q * ~~~~~~~~~~~~~~~~~a YJjt| z |j | ' ! ~~~~>EZ cr ~~ C(U(U (U~~~~~~~~~~~) cr (U co N N N (UCCC~~~~ .)0. C C o (U (UC ..C *_E~~~~~~~~~h E- E_ -, &,.aC.) 2 72 (U*C > > >. >. c C C ' ' ' C C X CE /)<<E< << <0 < J < 0 0. x 0 t- o (U (U(U1 C, C 0 C 0 N _ _ C C Z C C C C , .0 u C .' _ r E .2U CO C°' QC° E t > *Q°Ql-1 ._,_CC -= C tco co C) @UC C/) Cl) C') C/) C) U 976 c 0 ._5 0 0 u '-C Uc .0 E 0 -C :5 0.0_ 0 .0c m M (U .( i (U C x (U o E(U C (U 0 c , 0) b (U (U C-) C 0 . . E C 0 0. C C .C- CI .2_ (U (U C 0 V 0(U C(U D n 'a .2 0 0 C0 0 Q) c C 0) c c U cO g C (U CL 0. (A0. a) c Q (U)m CIL 0 (D(U zC E Q0.0 ,C(a CU _ A C + +3_ cc ._ (U U m n ._ 0 cm 0N-an (D c E ._ C(U cc .C %a 0 .5 0 a 0 E 0 C (U n(Um (U 0 , R0 C 0 0 E C C') OI 0 0 0 C 0 0C E 0 0(U c I- 5._CL 0. 0 -C I- 0 0 0 0 U)0 0 0 0 04) 4) 4) 4) 0 0 0 0 >_ >_ >_z >_ z>_ >_ >_ >_ 977MEDICINAL PLANTS IN THERAPY C .2 Cu 0 0 0 00 " C) 0 U 0~ ~ ~ 0 Cu 0 . C Cu CO Cu .o C .a > 30 . co Cu00W.W 00 t - 0 -- Cu V .) .0 00 0 U 0 Cu Cu~~~0 E CauC. cu x M:1~~ CuuC C o N N Cu.~~~~~~~~~ 0.0 . 0U~~~~~~~~~. co ~~~~~~0 co ''co c CL~~~~~~~~~~~~~~C C0 CL Cu C0C>C cm Cu.o.C 0 X uC ~~ E2 ~~ Eu uC.EE C +- Cu - u N. R. FARNSWORTH ET AL. Annex 2 Therapeutic indications of plant-derived drugs Therapeutic indication Drug Therapeutic indication Drug Abortifacient Analgesic Analeptic Antiarrhythmic Anticholinergic Antidepressant Antiemetic Antigout Antihepatotoxic Antihypertensive Anti-inflammatory Antioxidant Anti-Parkinsonism Antipyretic Antitussive Aphrodisiac Astringent Bronchodilator Capillary fragility Cardiotonic Trichosanthin Yuanhuacine Yuanhuadine Borneol Codeine Morphine Rotundine Salicin ( ±)-Tetrahydropalmatine Picrotoxin Quinidine Anisodamine Anisodine Atropine Hyoscyamine Glaziovine A9-Tetrahydrocannabinol Colchicine Silymarin Deserpidine Protoveratrines A & B Rescinnamine Reserpine Rhomitoxin Tetrandrine Aescin Borneol Bromelain Nordihydroguaiaretic acid L-Dopa Borneol Hemsleyadin Palmatine Quinine Bergenin Codeine Glaucine Noscapine Rorifone Yohimbine Hydrastine Khellin Theophylline Hesperidin Rutin Acetyidigoxin Adoniside Convallatoxin Deslanoside Digitalin Digitoxin Digoxin Gitalin Lanatosides A,B,C Ouabain Scillarin A Cerebral stimulant Chemotherapy: Anthelmintic Antiamoebic Antiascaris Antidysentery Antifungal Antimalarial Antitumour Choleretic Cholinesterase inhibitor Circulatory disorders CNS stimulant Condylomata acuminata Decrease ocular tension Dental plaque inhibition Detoxicant Diuretic Emetic Expectorant Haemostatic Insecticide Laxative Leukoderma Local anaesthetic Male contraceptive Oxytocic Parasympathomimetic Piscicide Vincamine Agrimophol Arecoline Quisqualic acid Emetine Glaucarubin Kainic acid Santonin Aesculetin Andrographolide Berberine Hemsleyadin Neoandrographolide Thymol Quinine Colchiceine amide Colchicine Demecolcine Etoposide Monocrotaline Teniposide e Vinblastine Vincristine Curcumin Cynarin Galanthamine Physostigmine Ajmalicine Caffeine Strychnine Podophyllotoxin A9-Tetrahydrocannabinol Sanguinarine Palmatine Theobromine Theophylline Emetine Pinitol (+ )-Catechin Hydrastine Nicotine Danthron Sennosides A & B Xanthotoxin Cocaine Gossypol Pachycarpine Sparteine Vasicine Pilocarpine Rotenone a Synthetic modification of a natural product. 978 MEDICINAL PLANTS IN THERAPY Annex 2: continued Therapeutic indication Drug Therapeutic indication Drug Proteolytic Respiratory stimulant Rubefacient Scabicide Sedative Skeletal muscle relaxant Smoking deterrent Smooth muscle relaxant Bromelain Chymopapain Papain oa-Lobeline Allyl isothiocyanate Camphor Menthol Methyl salicylate Benzyl benzoate Rotundine Scopolamine ( ± )-Tetrahydropalmatine Valepotriates Anabasine Cissampeline Tubocurarine a-Lobeline Papaverine Sweetener Sympathomimetic Tranquillizer Vasodilator Vitiligo Vulnerary Glycyrrhizin Phyllodulcin Stevioside Ephedrine Pseudoephedrine Pseudoephedrine, nor- Deserpidine Kawain Rescinnamine Reserpine Rhomitoxin Rotundine (± )-Tetrahydropalmatine Theobromine Xanthotoxin Allantoin Asiaticoside Annex 3 Plants used in traditional medicine and the drugs derived from them Plant' Drug Adhatoda vasica Vasicine Adonis vernalis Adoniside Aesculus hippocastanum Aescin Agrimonia eupatoria Agrimophol Ammi majus Xanthotoxin Ammi visnaga Khellin Anabasis aphylla Anabasine Ananas comosus Bromelain Anamirta cocculus Picrotoxin Andrographis paniculata Andrographolide Neoandrographolide Anisodus tanguticus Anisodamine Anisodine Areca catechu Arecoline Ardisia japonica Bergenin Artemisia maritima Santonin Atropa belladonna Atropine Berberis vulgaris Berberine Brassica nigra Allyl isothiocyanate Camellia sinensis Caffeine Theophylline Cannabis sativa A9-Tetrahydrocannabinol Carica papaya Chymopapain Papain See Annex 1 for plant authority names. Plant Drug Cassia acutifolia Cassia angustifolia Cassia species Catharanthus roseus Centella asiatica Cephaelis ipecacuanha Chondodendron tomentosum Cinchona ledgeriana Cinnamomum camphora Cissampelos pareira Citrus species Colchicum autumnale Convallaria majalis Coptis japonica Corydalis ambigua Crotalaria sessiliflora Curcuma longa Cynara scolymus Cytisus scoparius Daphne genkwa Sennosides A & B Sennosides A & B Danthron Vinblastine Vincristine Asiaticoside Emetine Tubocurarine Quinidine Quinine Camphor Cissampeline Hesperidin Rutin Colchiceine amide Colchicine Demecolcine Convallatoxin Palmatine ± )-Tetrahydropalmatine Monocrotaline Curcumin Cynarin Sparteine Yuanhuacine Yuanhuadine Annex 3: continued on next page 979 N. R. FARNSWORTH ET AL. Annex 3: continued Plant Drug Datura metel Digenia simplex Digitalis lanata Digitalis purpurea Ephedra sinica Erythroxylum coca Fraxinus rhynchophylla Gaultheria procumbens Glaucium flavum Glycyrrhiza glabra Gossypium species Hemsleya amabilis Hydrangea macrophylla var. thunbergii Hydrastis canadensis Hyoscyamus niger Larrea divaricata Lobelia inflata Lonchocarpus nicou Lycoris squamigera Mentha species Mucuna deeringiana Nicotiana tabacum Ocotea glaziovii Papaver somniferum Pausinystalia yohimba Scopolamine Kainic acid Acetyldigoxin Deslanoside Digoxin Lanatosides A, B, C Digitalin Digitoxin Gitalin Ephedrine Pseudoephedrine Pseudoephedrine, nor- Cocaine Plant Physostigma venenosum Pilocarpus jaborandi Piper methysticum Podophyllum peltatum Potentilla fragarioides Quisqualis indica Rauvolfia canescens Rauvolfia serpentina Aesculetin Rhododendron molle Methyl salicylate Rorippa indica Glaucine Salix alba Glycyrrhizin Sanguinaria canadensis Gossypol Silybum marianum Hemsleyadin Simarouba glauca Sophora pachycarpa Phyllodulcin Stephania sinica Hydrastine Stephania tetrandra Hyoscyamine Stevia rebaudiana Nordihydroguaiaretic acid Strophanthus gratus ai-Lobeline Strychnos nux-vomica Rotenone Theobroma cacao Galanthamine Thymus vulgaris Menthol Trichosanthes kirilowii L-Dopa Nicotine Urginea maritimaNic ne Valeriana officinalis Glaziovine Veratrum album Codeine Morphine Vinca minor Noscapine Several plants Papaverine Yohimbine Drug Physostigmine Pilocarpine Kawain Etoposide" Podophyllotoxin Teniposide b (+ )-Catechin Quisqualic acid Deserpidine Ajmalicine Rescinnamine Reserpine Rhomitoxin Rorifone Salicin Sanguinarine Silymarin Glaucarubin Pachycarpine Rotundine Tetrandrine Stevioside Ouabain Strychnine Theobromine Thymol Trichosanthin Scillarin A Valepotriates Protoveratrines A & B Vincamine Allantoin Benzyl benzoate Borneol Pinitol b Synthetic modification of a natural product. 980 MEDICINAL PLANTS IN THERAPY 981 to 0) oo 00 V- N N r LO ( 0NNr.:0) V. N: C N to (n N 0 .i)N N C N ) o~~~~~~~~~~~ C.) o~~~~~~~~~~~~3, 3 :3 Z 41 4- . 41 0 7 )5 05 _ C._) IC I u IC IC.) 0 E 0 0 0 .) 0 ll0V m 0 0 -o ._ 0 0 W.) 0 a.) C 0 0) -J .5 C 0 0._CL C 0 .4) .) 01)CD 0 U 0 .) .5 0 0 .0 m .0 0 00 0 7 - C.) 0 .0 C 0C. C0 C :O 0 4-I.) C._ 0 0 C.) 0 0. E D 0 0 0C.) 0:n 00 V 0 UD 0 .) 000 C 0 'Z'. C .0 0r C .0 C 6 o._< .0 0 0 0n 0 C 0 >S 0 X 0 0 >. 0 'C _i C 2E 0 Z = C 40 0. 0X. C0 .0 C 0)0 0 0 0. 0ifo 0 0 0 ._ 0 C 0 C _0 o C .0 wC C CC~ .' 4, ._ sV > C >°Cnoc s 0 >C .x Z, 0n 0J C = 7 0 0 C.> a a aX enc 0 a0 0) 0cr ,v-Ct' ci 6 o -"It -"* 1* Itt Nt LO Lo LoC~ o co ,t to 003 CV 't CV C.)0 m 0 0 C C.) 0 -c E o~~~~~~~~~~~~ C.) 0 0) 00 0 0 C.5 4-- 0 0 E 0 0 0. C1 . _ 0 .5 C 0 0 0. C .) C C. C 0 ._ .C -o C .5L C 0) 0 .5 0 0 c 0 -c 0 0) 0 .E_ 0 0. .) 0 C.) Z W 0 C 0 0 E 0 0 1. 0) CD 0w 0 *0 0 0 0 Z 0 *W 0 la 0 C 0 C 0 C.) 0 C C 0 .)_ : C 0 C C 0 ._ C.'0 0 0. C 0 0) 0 c. C.) C 0 0 C0 a; 0 C.) C C 0 C. 0 0 0 E E 0 4- C C 0 C0 .2 C 0 0 0 oW 0 0 0 .)_ C.) .t- W C 0 C.) :0 C 0 C.) ) .t ' 0 4) .EQ CN 0 .- t- 0 C.) __m0 0 0 4- cn ._ E E a) 4n U) U) C) C 0 0 a) ._ ) 0) .5 C 0 0._ C 0) 0 0 0 0 .0 0 Co 0) 0 0. 0 E CL 0 0 0C 0 0- C.) C .2_ c 0) - ._~~~~~~~~~~~~~~~~~~~0 0 0 n 'a -o 0 0 0 C._ Q u 0 0 c .) Q) C C 0 4) .5 0) C :3 U- C.)0 .' 0) C C b- C.) C0 C .)0 0 .LI C.) 0) .2 C C 0 ._ .2 .C CCC 0 .2 :t >- 0 N ._C .C -CC V C0 - C E 8 0 *U5 .CCin 0 0)C .C5 laC C 0 .0 C I 0 0 2 x 0 V0 . .2 - C .0 E CC C0 . 0) .- 0 C C a) C.) ._ 0 C.)-a 0i .C C aC 0 .0 .CC D .)_ 0 I a 0 '- 0 0 0 C0 40 0 0 0 C) W 0 0 CD 0 E I 0 L- .5 C.)0 0L C.):t .x0 040 U) m m 4) I. 0 u C5O m 41 E ol~ I t