-1 _bR 6ft EP1 ONCIIO. CIIfINOL PNOCRAMME RESEARGI REJ-OMINC SUB.IECP: @otaxonony of Sinnrlir:n d.amnosum oompl'ex rNsErflnroN! }[ro/oNGIo PERSONNEI: VAJIME Ch. PERIOD @VEREDT .0pri1 1979 - Febnra4r SIGNATURE OF RESPONSIBI,E OFFICffi: aaa a aaaaaoata @IITBfTS: - 1. Operations duri:rg portod. urrler review 2. I.[aterials 3. Results 4. Acknowled.gements RNSULTS - Sumnarised. in Table 1 - 7. Tab1e t (fiefa samples) Results from Southerrr Benirl - outside OCP zone. Table 2 (Ficld samplcs) Results from the 1tl! reimra.sion stlrd.ics in Togo and Benin Table 3 (Rearea material) Results from the 1979 reinvasion studios in Togo and Bcnin Table 4 (Field samples) Results from Ghana Table 5 (Reared material) Results from Ghana Table 6 (neared material) Results from some vrestern OCP areas. Table 7 (Fie1d ssnples) Results from llpper Vo1ta, I{a1i arrd Ivory Coast. OPERATIONS DURII\IG PERIOD TI}IDER BEITIIEI{ Anznra.L l-cave: April - Ilasr 1979. The period. und.er review covers April , 1979 to FebnrarSr, 1980. Work d.uring the periocl r^ras started. after ngr return from leave at the beginning of May. Crrtospecies id.ent if icat ion: l,laJy samplcs rcsulting from +he 1979 reinuasion studies in Togo ald Benin lrere receivetL in l{ay r,'rtrile outstarrding material frpm the lvory Coast, Gha.na arrd. Benin were being arralysed. Consoquently, as was the casc r,rith the previous report (.frfy D|B - I'[arch 1979) quarterry reports wero d.e]aycd in orrler to keep up with the vrork. Itre resulting dato ruas fortrarded st intenrals to the VC{I a1rfl. OCP collaboratorso Results presented. in this report thorefore represent combined. d.ata sruch of r.ihich has already been sent to those viro contributed. material for sturdgr. Trainine: - I{r. Fia-sorgbor (see report July 1978 - Marc}r 1979) contirmes to make good progress in thc techniques of chromosomal preparations ard intezpretation. Visit to IR0. Bouake In contirnration with collabcrative work,'with In0, I visited Borake in December. Dr. Quillevere ard. ryself d.iscussed. the c5rtospecies situation in western lvory Coast. i Dr. Od.etoyinbo (Ur*omologist, Feasibility stud.ies on Onchocerciasis Control in the Senegal River Basi.rr) who was also visiting IfiO drew ny attention to the request ho had made to the Programme Director concerning colraborative c5rtotaXonomic work j.:a the Senegal River Basin. },IATEBI.[Is - Reared, I.Iateria1 - FieId. sanpLes, T.ABLE 1 Results from Southern Benin - otrtside the OCP Ztone. L0 c TION Y I O}para Varia O}para Yawi O]<para 0kio Olcpara Iebadja 0ueme Oueme Zagttaltdo TtOtt Zo:e Zou 0ueme 0ueme Beffa 0ueme Terou Oueme Beterou I 1 3 4 5 6 7 9 10 11 12 13 14 15 15 17 >/ tzfiB t7 /6fie 1 11 - 2 3 2 4 18 13 26 7 1 5 , 7 4 3 19 21 18 3 26 6 3 18 12 I 13 6 3 IB 81 5 2 2 ll ll ll tt 1 6/t2fi8 7 /nfia il lf il 1 1 1 fi lt fi lt
T.ABLE 2 (ni.efa sampLes) RESUTTS FNOI'T IIIE 1979 REINVAS lON STTIDTNS I}I TOGO AI{D BUIIN t TION TE Y P TOGO. KPaaa Kpaza KDaza Kpaza Kpaza, Anie Anie Anie .Anie Anie Anie .0,nie Anie Anie anle/s.ltitta Assoukoko Itlono Mono Mono, Kpessi Gbarr Hou / l5oaii Gban Hou / l5oa5i Gban Hou / l5oa3f Gtarr Hou / l3oa5i Axagba I 1 2 3 4 3 Z 2 5E 68 5t, 5A 7 8 B 1 4 4 3 EO d.asq sa */>/rg 8/5fie zz/>ltg t/eftg zz/slrg zz/tltg t/5/tg g/sfig n il ll n il fl fi r ,! il n 23/5fie g/sfig zt/>lrg 4/5fie 1/efig 4 4 1 1 3 7 I 2 1 26 2 30 4 12 11 5 3 1 15 1 2 c 1 1 33 tt 2 1 2 2 11 11 1 3 18 14 4 11 11
Mono Llono Mono trnig Anie Anie Kpaza Kpaza Kpaza Akarna Koue Koue Koue KPaza I,{ono IIono Uono i,Iono Anie Kpouwa Aou Kolaware Na Trib. Karnasse 1 tspara 2 Kpaza 3 Bouza 5 TABTE 2 @I{TII\ru6 1e/6fie 1e/6fie 1e /G/1e n /6/1e N/6/1e n/s/tg n/6rte n/efig n/efig zt/6fi9 2,1/6fi9 zt/6f1e 21/6ne zt/5fie 12fi fie 12fi /1e 12/1fie 12fi fie tzlr fig 12fi ne Pn fie 12/1/19 tzfi /tg ttfi /tg t3/t /tg Blr fig 13fi fie YTO PECI 5 4 2 LO o T TO GO. 1 2 5 t 2 3 1 2 3 1 1 2 3 4 S1 3 11 14 13 7 21 3 tt 22 1B 1 18 55 19 2 1 4 ? 29 3 ? 1 2 6 7 B 9 5 3 4 13 2 2 5 1 7 1 I 6 5 D 9 17 1 9 3 24 5 2 1 4 5 35 3 saSg so da L T O G O. Kpei llare Konigbo I.lono below Tc]ramba Karnasse tri IIo TJDLE 2 @IYTI]'IUES Bfi fig 13/1fie 33 3 a 5 7 YTO 3 5 12 18 45 B 4 34 4 13 2 3 14 s1 4 6 B 24fi rte >/t /tg s/t trg z6/efie z6/6fie z6/5fie z6/6fie n /efig z6/6fie z6/G/re n /6/1e n /6fie n /5/1e n /e/tg n /efig 5 1 8 3EIIN. 0ueme Oueme 0ueme Oueme 0ueme llewe Beffa Zou Zou Okpara Besse Terou 3 4 6 B 11 5 7 9 10 12 13 14 1 1 2 2 3 5 7 2 3 1 1 1 tr I 4 2 1fuTerou at l,Iari{.Iaro a /sfrg 21 I Isol i sa
T.f.Bt4 :- (Reared l,laterial) RESULTS FNOI{ TIIE 1979 REINI/I,Src}I STTIDIES I}i TOCO II - JEIIIU 1 larva in Carnoy 12/6fi9 - ditto -B Ia:rtae in Carnoy 6/6fie - ditto -6 lanrae in Carno5r e/efig - ditto - Laicl egss zt/>ftg 6 batches 15 Lanrae in Ca:merY 6/6/19 uo a 146 t\/>/tg Laid eggs 21/5fi9 6 batches 4 lanrae in Carnoy 1o/6fi9 I,io a IIo Laid eggs z5/jtlg 7 batchesJ la.nrae in carnoy S/>/tg - ditto -1 larva in Carnoy - ditto -I lanrae in Carnoy 11/6ne - ditto - itto - axuae i:r CarnoS' 9 /6fi9 - tLitto -B larvae - ditto - 11 larvae in Carnoy A/dftg - ditto - 7 sirbarn:sl 1 sirbanum 3 sirbanum 1 sirbanum 8 sirbarum I s'irbanum 10 d.amnosum 3 sirbanum 1 s4ramosum ? 1 squarnosrrm I sqranrosunn 6 sqrramosum 2 damnosum 6 s4ramosum 2 darnnosum 1 sirbarrum 1 sqramosum 6 d.amnosurn -d31 - clitto - 11 l-arvae in Carnoy A/efig LOC ATI ON Keremou Keremou Keremou Zougou Zougou Zougou Wari ?Iari Hari I{a;ri Wari Wari Irlari I'lafo I.!aro I,laro I,laro llaro Itlaro l.Iaro Beterou I,Io a llo I[o a I,1o I[o a l.to I{o a IIo I,io a I[o Itlo a llo IIo a I,{o Ilo a I,1o I.Io Kamasse Confluence IIo Kanra,sse Confluence I,io I(amasse Corrf luence Mo Kanra.sse Confluence ],1o Kaoagse',&ff,luenoe I,lo Karnasse Conflucnce TfAtE 3 CO}IrINUES FIruTION DATE t/t lrg a/a fie afrfig t /t /tg 11/8fi9 13n fie 10/6fi9 zz/6fie zz/6fie zt/6fie 24/6lte z3/6/tg 23/6fie t/a fig 818fie 1/a/tg 1/Bfie afrfig e/afie 3tlt /tg t6/6fie n/6ne q/efig n /6/te tlr fig qfi fig t3/6fie 3o/6/7e 2. J sqpamosum 1 damnosum 2 sirbarrum 2 damnosum 1 dnmnosum '15 d.amnosun { d.amnosum J d.amnosurn 2 d.arnnosum J dsnnoflmj d.amnosum ) d.amnosum 2 damnosum .1O d.amnosum 3 d.amnosum { d.arnnosum 8 d.amnosum ! d.arnnosum 4 sirbamm 1 sirbarusl 2 sirbanum 1 sirbanum 2 sirbarun 1 sirbantrm 2 sirbanum 5 sirbarust 5 sirbarun 2 sirbanr:gl 7 sirbarn:gr 1 sqrarnosum 1 squarnosurn 2 sqranoaur 2 s4ramosum I s4rarrosum I sqramosum 5 squamosu.ur 12 squanosurt { s4ramosun J squamosum {. s4rarnos:m TABI,E 3 @NTINUES 3. Iro at lro D-n/tt/tg Laid esss zl/ttfig J batches 30 Larvae in Csrnoy Feredougoub a 'tZ/tZfi9 P J larvae in Carnoy Fereclougoub a lZ/12fi9 P 6 larrrae in Carnoy Basarr '1G17 /11f19 Laid esgs n/ltfig 5 Batches 30 lanrae i:n Carnoy Land.a Pozard.a 121 l+/1tfi9 Le.id esgs 15/11fi9 t batchcs 3O larvae in Carnoy s/s Karrdi n/+fig Iranc Itro 2 Feredougouba 1 z/lz/tg P 3 larwae in Carnoy Feredougoub a lZ/12fi9 P 2 larvae in Carnoy I,'eredougo.rb a I Z / I 2fi9 P J laruae in Carnoy Fercd.ougoub a lz/ lz/79 P { larvac in Carnoy 2 squamosum J d.arnnosum 2 d.amnosum 2 darnnosum Poor chromocomes Poor chromosomes Poor chromosomes Poor chromosomes Poor chromosomes Poor chromosomes Poor chromosomes TIISI,E 4: -RESULTS FROII GIJ,}IA LOCI,TIO}I Vea d.an Vea d.am Yendi d.anr R. Ku1pann, Yagaba Nasigr R, ]Ia.sia trlelwal R.Volta Odum-Anafar Br0durn Yaya Crossingr R.Yaya , F\rl1er Fsl.ls, R.Oyoko , nurpuffShi n. n rrpurn Aimasu strean, ilt inpokr.r Senchi Rapid.s I At ilrpolnr No mat ing betraviour Senchi Rapid.s Boti Falls Boti Falls Ainaso R; Arlqyeasc DATE CY'OSPECIES s/to fig e lto/te 11/10fi9 zz/t/8o z7 /tz/79 n /pfrg Bq ya si 1 1 11 5 6 11 da tah fig 13/ 10 fi9 31/5/1e t7 tr lrg ttfi /tg Efi fig 7 6 1 6 5 4 3 22 1 3 4 7 5 t /afig t /6fie 'tz/'tofi9 B/z/tg *,
TIBLE E (Reared l,Iaterial) RESULTS FNOI{ GtrII\NA soti falIs t7/8fi9 llep laja n/8/tg Single Batch lB5 larvae Boti falls t7 /Bh9 Egss laid n/\ltg Single Batch No 75. I lanrae Flies biting nun l/5/19 Pale ?Iing tr:ft Wegpe R. Dayi Eggs laid e/Sfig 1l lanrae in Caznoy 19/5/t9 Ditto 17 larwae in carnoy $/S/tg Hegpe z6/+fig dark Wing tuft Esss laid n/+figIn Carnoy ll/5fi9 FIies Biting nal ZG/+fi9 Blaelc Wing tuft.l l{egpe R. Dayi raid esss n /+/fg 18 larwae in Carnoy 15/5/19 Flies Bit ing ^ax, l/5fi9 PaIe ttring tuft, I.{egber R. Dayi Laid esss 6/5f19 20 la.:nrae in Carnoy l|/Sfitg Fliee Biting nan z6/qfi9 Blach Wing tuft.r- I{egpe; R. DayiLaid egss n/qfig\tl Larvae in Carnoy 16/5fi9 Flies fcd on ttan 3/5/t9 Pale l,Iing t.{t., Wegbe, R. Dayi Laid esss 5/5/79 10 laruae in Carnoy z5/5/tg Ditto 3 larnae in Carnoy 8/5fi9 Ditto ! larnae in Carnoy zt/S/tg Ditto 5 lanrae in Carnoy Zl/Sftg Flies fed on ltan 26/4fi9 Dark I'Iing tuftr. I"Ieg!e, R.Dayi Laid esss D /4fi9J larnae in Carnoy 21-23/jfi9 Poor chromosomes 2 squarnostrm ! squarnosum 8' sqrrarnosum 2 tahense 7 sanct ipauli 8 yahense 3, sanct ipauli 8 squarnosum 5 yahense 13 sanctipauli { s4ramosum Poor chromosomes 8 squamosum 6 sqramosum 4 yahense 1 sa.nct iparli TI3I,E 5_ EoLJTIIqJltr Fed on vtan 3o/4fi9 I.legPel R. Dayi Dgrk Wilg tuft r 8 batches Reared. using aerator or stirrer Commencing B/5fi9 34 larwaeIn Carnoy a:r,n 4/6/19 z5/5t 8/5, 1/6 '., a Bitins rran 21/5/t9 l,Iegbe, R. Dayi Pale $ling tuft, 2 batches Laid. egss z4/5fi9 13 lsrvae in'Carnoy lt/6fi9 Ditto 15 la.:rrrae in Carnoy 12 & 15/6fi9 tr'lies biting tran Z6/4fi9 Dark ttring tlftr WegPer R.Dayi Eeep laid. n /+/tg! larnae in barnoy 19/5fi9 Biting tx,art 21/5fi9 llegPel R. Dayi PaIe Wing tuftr 2 batchcs Lsid esss z4/5n9 1 1 larrae in Carnoy B, 9 , and. 9/e/t9. Sma-Il la:nrae thin chromosomes 11 sanct ipauli 12 squamosum 1d sq:.arnosum Poor chrorrsomes t sqrarnosum UEIE 6 (nearea l{aterial) RESULTS FNOU SOIIE TJESTMN OCP ^O,REAS. Leraba Brid.ge Leraba Brid.ge Karrkela I,ladina Diassa Kato Karrkela Iladina Diassa Kato D/Bfte 17 /B/Ie to/g /tg 25fi fie tsft/tg p/g trg 25fi /1e 15/B/1e 10 d.amnosr:n 27 cl.arnnosur,r 24 d.amnosum 6 d.amnosun t 14 sirbarnrm 10 d.arnnosum, 16 s4ramosum 1l darnnosum 12 damnosum, 19 sirbarnrm 5 d.a.nnosumr 13 Bqramosum T.aB!E-?s (rrmo seum,us) DATE sq !a CYTOSPECIES BA go cLaLOCATIO}I Yabo, VoIta Blanche Ouaga - Yarse Barnako, Sibi forest Bamako, Sibi Forest Nzo J R. ,Xo IIia 8 Ba:rou 1 Koba 4 ITemba 3 Cavally 10 Cavally 11 Souan 5 I ll n tt tl n n l? tt lt lt lt ll n 31fi fie 9 /8fi9 g tgfig t5fr fig zz/8fie o tBltg >/g lrg tz/g ltg 1e/e fie, z6/g ltg n/rcrte t7 /tofig leho/te 24/10/t9 3t /to fi9 a /e lte tz/g /tg tz/g ltg p/strg' g />/ts 30/5fie n />frg o /strg D /5/te tt/sfig tt/sfig to/sltg 7 1 )J 1 :J tr) 2 2 tr S1 10 12 2 15 6 10 3 6 lt 4 4 6 2 7 11 6 2 14 2 1 6 4 6 1
5CKTil LED I,{E}iT I arn gratcful to I,Ir. Akpoboua, Dr. Gauns Dr. Reiter (tttO Eonsr:Itarrts) ard members VCU staff ltro provided material for this I,lr. Fiasorgborts technical assistance is appreciated.. and of the worko great Iy