Detection of insecticide resistance in Aedes aegypti to organophosphate and synthetic pyrethroid compounds in the north-east of Thailand S. Pimsamarna, W. Sornpengb#, S. Akksilpb, P . Paepornc b and M. Limpawitthayakul a b
Department of Plants and Agricultural Resources, Khon Kaen University, Thailand
Office of Diseases Prevention and Control 7th, Ubon Ratchathani Province, Ministry of Public Health, Thailand c
National Institute of Health, Ministry of Public Health, Thailand
Abstract Aedes aegypti is a vector of dengue fever, which is a public health problem in the north-east of Thailand. During epidemics, emphasis is laid on the use of insecticides for the control of the disease. A survey of insecticides used was carried out in eight provinces, namely Amnat Charoen, Kalasin, Mukdahan, Nakhon Phanom, Sakon Nakhon, Si Sa Ket, Ubon Ratchathani and Yasothon. The study indicated that temephos was the only chemical used for the control of mosquito larvae while cypermethrin was used as space spray for the control of adult mosquitoes. Based on the results of resistance ratio of Ae. aegypti in comparison to the susceptible Bora Bora strain, Ae. aegypti showed greater resistance to deltamethrin and permethrin at a high level, with a 10-fold resistance ratio. Regarding the resistance in all study areas of Ae. aegypti to malathion and fenitrothion, it varied from low to medium levels. With biochemical assay, the results demonstrated that monooxygenase and esterase activity of Ae. aegypti had increased in all strains. Nevertheless, the glutathione-S-transferase activity had increased in seven strains. Therefore, monooxygenase, esterase and glutathione-S-transferase activity seems to be associated with mechanisms for creating pyrethroid-resistance in Ae. aegypti. These chemicals were used for the control of adult mosquitoes, which showed a high resistance ratio in these areas. Consequently, the continued use of these insecticides would lead to operational failure. Keywords: Aedes aegypti; insecticide; north-east Thailand; resistance.
Introduction Dengue fever, transmitted by Aedes aegypti, is a public health problem in the north-east of Thailand. Vector control by reducing #
Ae. aegypti population is at present the only viable option available to control the disease. Strategies to do so include environmental management and biological and chemical control. The north-east of Thailand has
E-mail: w_sornpeng@yahoo.com Dengue Bulletin – Volume 33, 2009
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relied mainly on the use of organophosphate and synthetic pyrethroids. During dengue epidemics, deltamethrin and cypermethrin were the main synthetic pyrethroids used to control adult Aedes mosquitoes through mass spraying,[1] which resulted in the vector’s resistance to pyrethroids. This is a worldwide phenomenon which is making control of vector-borne diseases difficult. The increase in the development of mosquito resistance is the primary concern of all control programmes. Common insecticideresistance mechanisms include alteration of target sites and increased enzyme activities of non-specific esterase, glutathione-S-transferase (GSTs) and P450-mediated monooxygenases. [2]
The major metabolic enzymes involved in resistance against pyrethroids in insects include P450-mediated monooxygenases, evaluated non-specific esterase, and reduced sensitivity of sodium ion channels along nerve axons. Recently, several cases of field–associated resistance have been reported in Ae. aegypti against pyrethroid products.[3] Resistance to pyrethroids, including permethrin and deltamethrin in Ae. aegypti, were documented in the north-east provinces of Thailand (see Figure). An attempt was made to lay bare the determinants, the level of resistance of Ae. aegypti to chemicals by susceptibility test, and study the changes in the level of enzymes that are related to the reduction and detoxification of chemical toxins of Ae. aegypti.
Figure: Map of study area in north-east Thailand
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Materials and methods Survey of insecticides Urban respondents from eight sites, Amnat Charoen, Kalasin, Mukdahan, Nakhon Phanom, Sakon Nakhon, Si Sa Ket, Ubon Ratchathani and Yasothon, were interviewed by using a questionnaire in a cross-sectional survey on the type of insecticides used to control larvae and adult Ae. aegypti. The key informants were the officials who were responsible for the control of contagious diseases at municipal offices and district administration offices in these areas. The survey took place between May and July 2007.
••
Resistance ratio (RR50) was calculated by comparing LT50 with LT50 of susceptible strain. LT50 resistance strain Resistance ratio, RR50 = LT50 susceptible strain*
Note: *The susceptible strain (Bora Bora) from the Faculty of Tropical Medicine, Mahidol University.
Biochemical assay Protein assay The total protein content of individual Ae. aegypti mosquitoes was determined using a commercial protein (Bio Rad) assay system. Results were compared with a derived standard curve. The plates were read after five minutes using an ELISA plate reader at 570 nm wavelength. Monooxygenase assay The procedure described by Valule et al. (1999)[6] was followed with only minor modifications. Fresh individual mosquitoes were homogenized in 50 ml distilled water in a 1 ml plastic vial. Homogenates were diluted with additional 150 µl distilled water. 20 µl of each homogenate was transferred to a microplate followed by the addition of 80 µl 0.0625 M potassium phosphate buffer (PPB) at pH 7.0. A solution of 0.01 g of 3,3’,5,5’-tetramethylbenzidine (TMBZ) in 5 ml methanol was prepared and a 0.25 M sodium acetate buffer (pH 5.0) was added. Following this, 200 µl of TMBZ solution was added with the 100 µl of mosquito homogenate plus PPB in each well followed by 25 µl of 3% hydrogen peroxide. The plates were read after 5 and 10 minutes using an ELISA plate reader at 620 nm wavelength.
Study of resistance to insecticides Testing of susceptibility of Ae. aegypti to chemicals was carried out and the results were interpreted, as recommended by the World Health Organization.[4] The adult mosquitoes used were reared from larvae collected from the study areas to generation F1. Fully-grown females aged 3–5 days were fed on 5% sugar before testing, and then placed inside an exposure tube that was lined with chemicallytreated paper. Each tube contained 25 mosquitoes and there were four tubes in all. The mosquitoes were left in the tubes for 15, 30, 60 and 120 minutes with the temperature at 25±2 °C. After the exposure time, the test mosquitoes were transferred to the holding tubes and cotton pad soaked in 10% sugar solution was provided. The mortality was recorded after 24 hours. •• Interpretation of the results of the bioassay data were pooled and LT50 was obtained by probit analysis.[5]
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Esterase assay In the method of Peiris and Hemingway (1990), [7] the test could begin by adding 30 mM -naphthyl acetate that had been previously prepared at a fixed quantity in each well and leaving it at a temperature of 30 °C for 15 minutes. Following that, 0.1% fast blue B-SDS was added and then a 20 µl sample of the mosquito that had been separated was added to each well and it was left at room temperature at 25±2 °C. The test was then carried out using a microplate reader and the absorbance value was read at 620 nm. Glutathione-S-transferase (GST) assay GST activity was assayed following Habig et al. (1974).[8] The working solutions were mixed at a ratio of 125 µl CDNB to 2.5 ml GSH. In the test, 20 µl of the prepared liquid was added to each well. Following that, a 20 µl sample of the mosquito that had been separated was
added to each well and it was left at room temperature (25±2 °C) for 15 minutes. The test was then carried out using a microplate reader and the absorbance value was read at 340 nm. The analysis was carried out using ANOVA, which was used to compare the protein content and enzyme expression levels within and between populations. All levels of statistical significance were determined at 95% confidence limit.
Results Survey of insecticides A survey indicated that the only chemical used in controlling mosquito larvae was temephos while the chemical most commonly used in spraying to control adult mosquitoes was cypermethrin (Table 1).
Table 1: Types of chemicals used in all eight study provinces Province Amnat Charoen Number of offices 22 Chemical used in controlling larvae Temephos Per cent used 100 Chemical used in controlling adult mosquitoes Cypermethrin Zetamethrin+ Dichlorvos Total Kalasin 69 Temephos 100 100 Total Cypermethrin Alpha-cypermethrin Zeta-cypermethrin Deltamethrin Etofenprox Zetamethrin+ Dichlorvos Total 100 Total Per cent used 95.45 4.55 100 71.01 1.45 1.45 5.80 1.45 18.84 100
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Province Mukdahan
Number of offices 21
Chemical used in controlling larvae Temephos Total
Per cent used 100 100 100
Chemical used in controlling adult mosquitoes Cypermethrin Total Cypermethrin Deltamethrin Zetamethrin + Dichlorvos
Per cent used 100 100 90.91 6.06 3.03 100 90.90 6.06 1.52 1.52 100 93.18 2.27 1.14 1.14 1.14 1.14 100 88.89 2.47 2.47 3.70 1.23 1.23 100 87.72 8.77 1.75 1.75 100
Nakorn Phanom
33
Temephos
Total Sakon Nakorn 66 Temephos
100 100
Total Cypermethrin Deltamethrin Etofenprox Zetamethrin + Dichlorvos
Total Si Sa Ket 88 Temephos
100 100
Total Cypermethrin Alpha-cypermethrin Bifenthrin Dichlorvos Fenitrothion Sumithion
Total Ubon Ratchathani 81 Temephos
100 100
Total Cypermethrin Alpha-cypermethrin Zeta-cypermethrin Deltamethrin Fenitrothion Etofenprok
Total Yasothon 57 Temephos
100 100
Total Cypermethrin Alpha-cypermethrin Zeta-cypermethrin Dichlorvos
Total
100
Total
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Resistance level of Ae. aegypti to insecticides The results of the study of Ae. aegypti’s resistance level to insecticides are included in Table 2. It may be seen that Ae. aegypti, in every area, showed high resistance to deltamethrin and permethrin. Ae. aegypti from Si Sa Ket had the highest resistance ratio to 0.05% deltamethrin at 24.80-fold. Next in order were Ae. aegypti from Mukdahan, Ubon Ratchathani and Sakon Nakhon with resistance ratios of 23.96-, 22.78-, and 20.91-fold respectively. With regard to the study of the resistance rate to treated paper with 0.75% permethrin, Ae. aegypti from Kalasin had the highest resistance ratio at
15.13-fold. Regarding the chemicals, namely 5% malathion and 1% fenitrothion, Ae. aegypti from all areas had resistance ratios that varied from low to medium.
Biochemical study The results of testing the amount of proteins and enzymes in Ae. aegypti in all eight provinces are included in Table 3. The investigations indicated that the monooxygenase, esterase and glutathione-S-transfease enzymes had shown a tendency to increase when compared with the Bora Bora susceptible strain. Nevertheless, the glutathione-S-transferase activity had shown an increasing trend in seven strains.
Table 2: Resistance ratios of the eight strains of Ae. aegypti to four types of chemically-treated paper in the north-east of Thailand Chemicals Ae. aegypti strains 0.5% deltamethrin LT50* (minute) Bora Bora Amnat Charoen Kalasin Mukdahan Nakhon Phanom Sakon Nakhon Si Sa Ket Ubon Ratchathani Yasothon 1.0821 21.9124 15.9024 25.9294 21.5179 22.6224 26.8370 24.6451 20.4058 1 20.25 13.95 23.96 19.88 20.91 24.80 22.78 18.86 RR50** 0.75% permethrin LT50 (minute) 6.3388 67.0799 95.8781 80.6522 68.3064 70.8879 64.6004 74.2632 64.3992 1 10.58 15.13 12.72 10.78 11.18 10.19 11.72 10.16 RR50 5% malathion LT50 (minute) 2.7756 21.6527 12.4080 17.8287 14.3295 16.5196 13.8454 10.0989 6.7857 1 7.80 4.47 6.42 5.16 5.95 4.99 3.64 2.44 RR50 1% fenitrothion LT50 (minute) 5.9366 25.5521 19.6806 25.1353 21.7699 28.9672 27.6615 9.1136 11.5399 1 4.30 3.32 4.23 3.67 4.88 4.66 1.54 1.94 RR50
* the LT50 value of the Ae. aegypti to chemically-treated paper. ** the resistance ratio (RR50) = LT50 field strain / LT50 susceptible strain.
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Table 3: Levels of proteins and enzymes for all eight strains of Ae. aegypti in the north-east of Thailand when compared with susceptible strain Strains (n=40 mosquitoes for each) Bora Bora Amnat Charoen Kalasin Mukdahan Nakhon Phanom Sakon Nakhon Si Sa Ket Ubon Ratchathani Yasothon Total protein Mean±SD (mg protein/ml per mosquito (n)) 0.6569+0.0919 c* 0.8252+0.0952 ab 0.6747+0.0936 bc 0.8604+0.1340 a 0.8613+0.1254 a 0.8236+0.1311 ab 0.7143+0.1386 b 0.6891+0.1129 bc 0.7774+0.1008 b MFOs (nmol product/min/ mg protein) 0.0561+0.3552 c 0.1285+0.1174 bc 0.1054+0.0646 b 0.3846+0.1913 a 0.2257+0.1623 bc 0.1184+0.0957 b 0.0573+0.0139 a 0.0697+0.0446 a 0.1331+0.0927 bc Esterase (nmol naphthol/ min/mg protein 0.9175+0.6176 c 1.4279+0.2225 a 1.3357+0.1307 b 1.3962+0.1315 b 1.3409+0.1234 b 1.3970+0.0815 b 1.5420+0.0498 a 1.4553+0.0844 a 1.3223+0.1346 b GSTs (nmol CDNB/min/ mg protein) 0.9701+0.7335 c 1.2642+0.0481 b 0.9832+0.0824 c 1.4897+0.0843 a 1.4941+0.0614 a 0.9577+0.0741 c 1.1241+0.0348 b 1.1076+0.0357 b 1.2661+0.0249 b
*In every column that ends with the same letter, there is no statistical difference from a comparison using the Duncan multiple range test at a confidence level of 95%.
Discussion Dengue fever has been a public health problem in the north-east of Thailand for more than 50 years. Control of the disease in the epidemic season warrants the use of chemicals to eliminate the Ae. aegypti vector mosquito both at the larval and adult stages. Temephos 1% has been recommended for larval control because of its low oral toxicity in mammals (8600 mg/kg in male rats), and it is one of the few insecticides recommended for potable water.[9] The chemical that was mostly used to spray adult mosquitoes was cypermethrin. The present study on the level of resistance of Ae. aegypti in all eight provinces, in comparison with that of the Bora Bora susceptible strains, indicated high resistance to deltamethrin and permethrin. [10] The
resistance ratio was found to be more than ten-fold.[11] With regard to other chemicals, viz. malathion and fenitrothion, the resistance ratio in Ae. aegypti varied from low to medium. Sathantriphop,[12] who studied the resistance phenomenon to various insecticides from four groups in a field strain of Ae. aegypti in the Baan Suan community, in Nonthaburi province, Thailand, also detected its high resistance to deltamethrin and permethrin but the vector was 100% susceptible to fenitrothion. The results of the study by biochemical assay of the amount of protein, monooxygenase, esterase and glutathione-S-transfease enzyme had shown a tendency to increase when compared with the Bora Bora susceptible strain. This suggested that monooxygenase, esterase and glutathione-S-transfease were
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associated with permethrin and deltamethrin resistance in Ae. aegypti . Resistance to insecticides usually appears to be unstable, and is associated with a genetic cost in the absence of selection.[13,14] This has occurred due to other resistance-creating mechanisms apart from the increase of enzymes that detoxify the aforementioned insecticidal toxins. Regular monitoring of the mosquitoes’ susceptibility to the most widely used insecticides is necessary to ensure an appropriate choice of chemicals. Introduction of inappropriate insecticides without a proper understanding of
the prevailing resistance mechanisms may lead to operational control failure. Moreover, early detection and knowledge of the resistance status as well as the underlying mechanisms in vector mosquitoes are essential for effective long-term control of Ae. aegypti.
Acknowledgements This research was supported in part by the graduate school, Khon Kaen University, and the WHO Country Office, Thailand.
References [1] Department of Communicable Disease Control, Ministry of Public Health, Thailand. Vector borne disease annual report 2002-2003. Nonthaburi: Bureau of Vector Borne Diseases, 2003. [2] Hemingway J, Ranson H. Insecticide resistance in insect vectors of human disease. Annual Review of Entomology 2000;45:371-91. [3] Scott JA. The molecular genetics of resistance: resistance as a response to stress. Symposium on pesticide resistance. Insect biochemistry and molecular biology 1999;29:757-77. [4] World Health Organization. Dengue hemorrhagic fever: diagnosis, treatment and control. Geneva, 1986. [5] Finney JD. Probit analysis. 3rd ed. Cambridge: The University Press, 1971. [6] Vulule JM, Beach RF, Atieli FK, McAllister JC, Brogdon WG, Roberts JM, et al. Elevated oxidase and esterase levels associated with permethrin tolerance in Anopheles gambiae from Kenyan villages using permethrin impregnated nets. Medical Veterinary and Entomology 1999;13:239-44. [7] Peiris HTR, Hemingway J. Temephos resistance and the associated cross resistance spectrum in a strain of Culex quinquefasciatus from Peliyagoda, Sri Lanka. Bulletin of Entomological Research 1990;80:49-55. [8] Habig WH, Padst MJ, Jakody WB. Glutathione S-transferase the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry 1974;249(22):7130-9. [9] World Health Organziation, Regional Office for the Pan American. Dengue and dengue hemorrhagic fever in the Americas: guidelines for prevention and control. Washington, DC: PAHO, 1994. pp. 548. [10] Paeporn P, Supaphathom K, Srisawat S, Boonyabancha, Phanurai P . Efficacy of aerosol insecticide product. Bull Dept Med Sci 1996;38:37-43. [11] Mazzari MB, Georghiou GP . Characterization of resistance to organophoshate, carbamate, and pyrethroid insecticides in field populations of Aedes aegypti from Venezuela. Journal of the American Mosquito Control Association 1995;11:315-22.
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[12] Sathantriphop S, Paeporn P , Supaphathom K. Detection of insecticides resistance status in Culex quinquefasciatus and Aedes aegypti to four major groups of insecticides. Tropical Biomedicine 2006;23(1):97-101. [13] Hemingway J, Hawkes NJ, McCarroll L, Ranson H. The molecular basis of insecticide resistance in mosquitoes. Insect biochemistry and molecular biology 2004;34:653-65.
[14] World Health Organization. Instructions for determining the susceptibility or resistance of adult mosquitoes to organochlorine, organophosphate and carbamate insecticidediagnostic test. Document no. WHO/ VBC/81-806. Geneva: WHO, 1981.
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